Summary of Study ST004271

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002697. The data can be accessed directly via it's Project DOI: 10.21228/M81R8T This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST004271
Study TitleMetabolomic profiles of sweet potato (Ipomoea batatas) cultivars: Insights into biochemical differences
Study SummarySweet potato (Ipomoea batatas L.) is regarded as a crucial crop for enhancing food security. The crop offers several opportunities to enhance agricultural productivity, particularly in developing nations, for small-scale farmers. Greater emphasis has been made on sweet potato studies due to the crop’s nutritional value, including metabolites which are important in the plants’ defence system. Metabolomics has become a useful tool for improving our understanding of primary and secondary metabolism in plants. Hence, the current study aimed at using metabolomics tools to determine biochemical differences between four different sweet potato cultivars (Atacama, Jane, Blesbok and Bellevue) grown in South Africa. An untargeted metabolomics approach ,UHPLC-qTOF MS, was used to assess the metabolite profiles of the four cultivars. Biochemical analysis identified metabolites belonging to various classes, with flavonoids being the most significantly enriched class. Key metabolites included kaempferol 7,4’-dimethyl ether 3-O-sulfate, 7-hydroxy-3-methylflavone, myricetin 3,3’-digalactoside, 7β,16α-diacetoxywithanolide D and myristoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine. The results from this study also indicate differential expression of these metabolites which could be due to cultivar specific pathways that may be influenced by genetics, environmental factors and stress tolerance introduced through plant breeding . This study provides insights into the metabolic differences of sweet potato cultivars, which offers potential biomarkers contributing towards crop improvement.
Institute
Nelson Mandela University
Last NameRauwane
First NameMolemi
AddressUniversity Way, Summerstrand, Gqeberha, 6019
Emailmolemi.rauwane@mandela.ac.za
Phone+27415044554
Submit Date2025-09-26
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-10-30
Release Version1
Molemi Rauwane Molemi Rauwane
https://dx.doi.org/10.21228/M81R8T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002697
Project DOI:doi: 10.21228/M81R8T
Project Title:Metabolomic profiles of sweet potato (Ipomoea batatas) cultivars
Project Summary:In the current syudy, four distinct sweet potato cultivars—Atacama, Jane, Blesbok, and Bellevue—grown in South Africa were compared biochemically using metabolomics methods. UHPLC-qTOF MS, an untargeted metabolomics technique, was used to evaluate the metabolite profiles of the four cultivars.
Institute:Nelson Mandela University
Last Name:Rauwane
First Name:Molemi
Address:University Way, Summerstrand, Gqeberha, 6019
Email:molemi.rauwane@mandela.ac.za
Phone:+27415044554
Funding Source:Water Research Commission (Project No. C2023/2024-01262)

Subject:

Subject ID:SU004424
Subject Type:Plant
Subject Species:Ipomoea batatas
Taxonomy ID:4120
Gender:Not applicable

Factors:

Subject type: Plant; Subject species: Ipomoea batatas (Factor headings shown in green)

mb_sample_id local_sample_id Cultivar Sample source
SA497764Figlan_22042024_S7Atacama leaves
SA497765Figlan_22042024_S2Atacama leaves
SA497766Figlan_22042024_S8Atacama leaves
SA497767Figlan_22042024_S1Atacama leaves
SA497768Figlan_22042024_S6Atacama leaves
SA497769Figlan_22042024_S4Atacama leaves
SA497770Figlan_22042024_S3Atacama leaves
SA497771Figlan_22042024_S5Atacama leaves
SA497772Figlan_22042024_S80Bellevue leaves
SA497773Figlan_22042024_S79Bellevue leaves
SA497774Figlan_22042024_S78Bellevue leaves
SA497775Figlan_22042024_S77Bellevue leaves
SA497776Figlan_22042024_S76Bellevue leaves
SA497777Figlan_22042024_S75Bellevue leaves
SA497778Figlan_22042024_S74Bellevue leaves
SA497779Figlan_22042024_S73Bellevue leaves
SA497780Figlan_22042024_S54Blesbok leaves
SA497781Figlan_22042024_S56Blesbok leaves
SA497782Figlan_22042024_S55Blesbok leaves
SA497783Figlan_22042024_S52Blesbok leaves
SA497784Figlan_22042024_S53Blesbok leaves
SA497785Figlan_22042024_S51Blesbok leaves
SA497786Figlan_22042024_S50Blesbok leaves
SA497787Figlan_22042024_S49Blesbok leaves
SA497788Figlan_22042024_S32Jane leaves
SA497789Figlan_22042024_S31Jane leaves
SA497790Figlan_22042024_S30Jane leaves
SA497791Figlan_22042024_S29Jane leaves
SA497792Figlan_22042024_S28Jane leaves
SA497793Figlan_22042024_S27Jane leaves
SA497794Figlan_22042024_S26Jane leaves
SA497795Figlan_22042024_S25Jane leaves
Showing results 1 to 32 of 32

Collection:

Collection ID:CO004417
Collection Summary:Sweet potato trials were carried out at the Agricultural Research Council, Roodeplaat- Vegetable, Industrial and Medicinal Plants Research Institute (ARC-VIMP), Pretoria, South Africa (25 ̊59” S, 28 ̊35” E; 1189 m altitude). Experimental trials were conducted during February 2024 to April 2024. Cuttings were obtained from greenhouse propagation at the ARC-VIMP, whereby the cultivars Atacama, IIAM-Jane, Bellevue and Blesbok were pre-selected for the study. Each cultivar was cut approximately 30 cm long and planted three nodes below the soil surface. Plants were assembled in four replicates of a randomized complete block design (RCBD). Plants were watered once a week to field capacity and fertilized with multifeed and LAN (28) when required. Harvested leaf material were pulverised using liquid nitrogen and 200 mg of the powdered material was transferred into a 2 ml centrifuge tube.
Collection Protocol Filename:Sweetpotato_protocol.pdf
Sample Type:Leaves
Collection Location:Agricultural Research Council- Roodeplaat Vegetable and Ornamental Plants
Storage Conditions:-80℃

Treatment:

Treatment ID:TR004433
Treatment Summary:No treatment
Plant Growth Location:Agricultural Research Council- Vegetable, Industrial and Medicinal Plant, Roodeplaat, South Africa
Plant Plot Design:242 m2
Plant Estab Date:2024-02-05
Plant Harvest Date:2024-04-03
Plant Harvest Method:Young leaves were harvested in 50ml tubes and snapped frozen in liquid nitrogen
Plant Storage:-80C

Sample Preparation:

Sampleprep ID:SP004430
Sampleprep Summary:Harvested leaf material were pulverised using liquid nitrogen and 200 mg of the powdered material was transferred into a 2 ml centrifuge tube. Sample extraction process was carried using a modified methodology outlined by Maserumule et al.,2023. A1.5 ml of 80% ice-cold methanol (HPLC grade, Minema Chemicals) was added to each sample, and the mixture was vortexed for 30 seconds. Samples were sonicated for 2 hours in 4˚C cold water, and then centrifuged for 5 minutes at 2850 ref per minute (rpm) at 4°C. The supernatant was then transferred to a 2 ml tube and stored at 4°C. Filtration of extracted samples was performed using 0.22 µm nylon filters into glass vials containing 500 µl inserts (Alwsci Techologies, 6x31 mm). Maserumule M, Rauwane M, Madala NE, Ncube E, Figlan S. 2023 Defence-related metabolic changes in wheat (Triticum aestivum L.) seedlings in response to infection by Puccinia graminis f. sp. tritici. Front Plant Sci 14. (doi:10.3389/fpls.2023.1166813)
Sampleprep Protocol Filename:Sweetpotato_protocol.pdf
Processing Storage Conditions:Described in summary
Extract Storage:4℃

Chromatography:

Chromatography ID:CH005401
Methods Filename:Sweetpotato_protocol.pdf
Instrument Name:Shimadzu LCMS-9030
Column Name:Shim pack Velox C18 (100 x 2.1 mm, 2.7 um)
Column Temperature:55
Flow Gradient:2 minutes 10% B equilibration; 10–60% B induced throughout 3–5 minutes. From 5-8 minutes, the conditions were altered from 60–90% B, and from 8–11 minutes, the gradient was maintained at 90%. At one minute (11-12 minutes), the gradient was returned to initial conditions of 90-60% at 1 minute, which was followed by a 1-minute column equilibration period
Flow Rate:0.45 ml/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN007109
Analysis Type:MS
Analysis Protocol File:Sweetpotato_protocol.pdf
Operator Name:Prof Ntakadzeni Madala
Chromatography ID:CH005401
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST004271_AN007109_Results.txt
Units:peak area
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