Summary of Study ST004274
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002700. The data can be accessed directly via it's Project DOI: 10.21228/M8NK11 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004274 |
| Study Title | Loss of vitamin C biosynthesis protects from the pathology of a Schistosome infection and egg production. |
| Study Type | Treatment experiment |
| Study Summary | The processes influenced by ascorbate treatment in schistosome reproduction were investigated by metabolomics analysis of Ascorbate-treated and -untreated worm samples in the culture media. The top metabolite enriched in ascorbate-treated schistosomes was L-DOPA, a product of tyrosinase activity, which crosslinks vitellocyte proteins to form the schistosome eggshell, consistent with a role for ascorbate in vitellocyte development. |
| Institute | University of Texas Southwestern Medical Center at Dallas |
| Department | Children’s Medical Center Research Institute |
| Laboratory | Michalis Agathokleous |
| Last Name | Agathokleous |
| First Name | Michalis |
| Address | 6000 Harry Hines Blvd, NL12.110N, Dallas, TX, 75235 |
| michail.agathokleous@utsouthwestern.edu | |
| Phone | 2146486270 |
| Submit Date | 2025-10-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-10-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002700 |
| Project DOI: | doi: 10.21228/M8NK11 |
| Project Title: | Loss of vitamin C biosynthesis protects from the pathology of a Schistosome infection |
| Project Type: | MS quantitative analysis |
| Project Summary: | Schistosoma parasite is known to require ascorbate to produce eggs in vitro culture. To investigate the effect of ascorbate deficiency in mice infected with Schistosoma mansoni, the pathology of infection was analyzed in vivo. S. mansoni required host ascorbate to produce eggs and consequently, ascorbate-deficient mice were protected from schistosomiasis pathologies including liver granuloma formation and transmission. To understand the processes dependent on ascorbate metabolome and gene expression profiles were compared between Ascorbate-treated and -untreated samples. Our work shows that ascorbate deficiency can have physiological benefits by protecting animals from the pathology of a major parasitic disease. |
| Institute: | UT Southwestern Medical Center |
| Department: | CRI |
| Laboratory: | Michalis Agathokleous |
| Last Name: | Agathokleous |
| First Name: | Michalis |
| Address: | 5323 Harry Hines Blvd, Children's Research Institute, Dallas, TX, 75309, USA |
| Email: | michail.agathokleous@utsouthwestern.edu |
| Phone: | 2146486270 |
Subject:
| Subject ID: | SU004427 |
| Subject Type: | Other organism |
| Subject Species: | Schistosoma mansoni |
| Taxonomy ID: | 6183 |
Factors:
Subject type: Other organism; Subject species: Schistosoma mansoni (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype | Treatment | Sample source | Injection order |
|---|---|---|---|---|---|
| SA497868 | Asc_5 | wild type | Ascorbate | Culture | 10 |
| SA497869 | Asc_1 | wild type | Ascorbate | Culture | 6 |
| SA497870 | Asc_2 | wild type | Ascorbate | Culture | 7 |
| SA497871 | Asc_3 | wild type | Ascorbate | Culture | 8 |
| SA497872 | Asc_4 | wild type | Ascorbate | Culture | 9 |
| SA497873 | dep_1 | wild type | Control | Culture | 1 |
| SA497874 | dep_2 | wild type | Control | Culture | 2 |
| SA497875 | dep_3 | wild type | Control | Culture | 3 |
| SA497876 | dep_4 | wild type | Control | Culture | 4 |
| SA497877 | dep_5 | wild type | Control | Culture | 5 |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO004420 |
| Collection Summary: | For metabolomics, 5 worm pairs were cultured in 12-well plates of ABC169 media, with or without ascorbate for 24 hours. To separate worm pairs, worms were suspended in BM169 with 0.25% tricaine and agitated for 5 min. Unpaired female worms were washed with cold PBS two times and snap-frozen in liquid nitrogen. |
| Sample Type: | Whole worm lysate |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR004436 |
| Treatment Summary: | 5 worm pairs isolated from infected wild type mice were cultured in 12-well plates of ABC169 media, with or without ascorbate for 24 hours. |
Sample Preparation:
| Sampleprep ID: | SP004433 |
| Sampleprep Summary: | Metabolites were extracted by adding 30 μl of chilled 40:40:20 methanol:acetonitrile:water (v:v:v) solvent to five female worms. The samples were ground with a pestle, sonicated for 1 min, vortexed for 1 min and centrifuged at 20,000 × g for 15 min at 4 °C. For metabolomics analysis, the supernatant was transferred to a new tube, centrifuged at 21,000 × g for 15 min at 4 °C and transferred to LC-MS autosampler vials. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Chromatography:
| Chromatography ID: | CH005404 |
| Instrument Name: | Thermo Scientific QExactive HF-X hybrid quadrupole orbitrap high-resolution mass spectrometer (HRMS) |
| Column Name: | Millipore ZIC-pHILIC column (150 x 2.1 mm, 5 μm) |
| Column Temperature: | 40 |
| Flow Gradient: | 0–15 min linear ramp from 90% B to 30% B; 15–18 min isocratic flow of 30% B; 18–19 min linear ramp from 30% B to 90% B; 19–27 column regeneration with isocratic flow of 90% B. |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% water; 10 mM ammonium acetate, pH 9.8 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN007113 |
| Analysis Type: | MS |
| Chromatography ID: | CH005404 |
| Num Factors: | 10 |
| Num Metabolites: | 274 |
| Units: | area under curve |
