Summary of Study ST004279

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001984. The data can be accessed directly via it's Project DOI: 10.21228/M88J0W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST004279
Study TitleMetabolic aging in human pancreas
Study SummaryTo determine whether age-associated metabolites identified in our metabolic atlas of mouse aging could be validated in human tissue, we collected human pancreas samples from donors at the Gift of Life Michigan Donor Care Center. The samples we acquired included pancreases from males and females spanning a broad age range allowing us to investigate how age and sex affect pancreas metabolism. We used a targeted ms/ms approach to accurately identify 96 metabolites across the data set. We found that hydroxyproline, which decreases with age in mouse pancreases, also decreases with age in human pancreases.
Institute
University of Southern California
DepartmentKeck School of Medicine
LaboratoryMullen Lab
Last NamePilley
First NameSteven
Address1501 San Pablo St., Los Angeles, CA, 90033, USA
Emailpilley@usc.edu
Phone+13234427555
Submit Date2025-08-25
Num Groups1
Total Subjects34
Num Males27
Num Females7
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-11-19
Release Version1
Steven Pilley Steven Pilley
https://dx.doi.org/10.21228/M88J0W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001984
Project DOI:doi: 10.21228/M88J0W
Project Title:A metabolic atlas of mouse aging
Project Summary:Humans are living longer, but this is accompanied by an increased incidence of age-related chronic diseases. Many of these diseases are influenced by age-associated metabolic dysregulation, but how metabolism changes in multiple organs during aging in males and females is not known. Answering this could reveal new mechanisms of aging and age-targeted therapeutics. In this study, we describe how metabolism changes in 12 organs in male and female mice at 5 different ages. Organs show distinct patterns of metabolic aging that are affected by sex differently. Hydroxyproline shows the most consistent change across the dataset, decreasing with age in 11 out of 12 organs investigated. We also developed a metabolic aging clock that predicts biological age and identified alpha-ketoglutarate, previously shown to extend lifespan in mice, as a key predictor of age. Our results reveal fundamental insights into the aging process and identify new therapeutic targets to maintain organ health.
Institute:University of Southern California
Department:Keck School of Medicine
Laboratory:Mullen Lab
Last Name:Pilley
First Name:Steven
Address:1501 San Pablo St., Los Angeles, CA, 90033, USA
Email:pilley@usc.edu
Phone:+13234427555
Funding Source:Impetus Grants 014746-00001

Subject:

Subject ID:SU004432
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:20s-70s
Gender:Male and female
Human Race:White, African American

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Age Sex Race Injection order
SA498253Blank-GiftOfLife- - - - 1
SA498254Gift of Life Pool 2- - - - 15
SA498255Cell QC-GiftOfLife- - - - 2
SA498256Gift of Life Pool 3- - - - 26
SA498257Gift of Life Matrix Blank- - - - 3
SA498258Gift of Life Pool 1- - - - 4
SA498259Gift of Life Pool 4- - - - 41
SA4982601_HPPancreas 20-29 Male African American 13
SA4982614_HPPancreas 20-29 Male African American 33
SA4982626_HPPancreas 20-29 Male African American 37
SA4982633_HPPancreas 20-29 Male White 19
SA4982645_HPPancreas 20-29 Male White 20
SA4982652_HPPancreas 20-29 Male White 30
SA4982668_HPPancreas 30-39 Female White 16
SA49826710_HPPancreas 30-39 Female White 21
SA49826811_HPPancreas 30-39 Female White 29
SA4982699_HPPancreas 30-39 Female White 6
SA4982707_HPPancreas 30-39 Male African American 17
SA49827115_HPPancreas 30-39 Male African American 28
SA49827212_HPPancreas 30-39 Male White 23
SA49827314_HPPancreas 30-39 Male White 24
SA49827413_HPPancreas 30-39 Male White 8
SA49827516_HPPancreas 40-49 Female African American 32
SA49827617_HPPancreas 40-49 Female White 40
SA49827722_HPPancreas 40-49 Male White 14
SA49827820_HPPancreas 40-49 Male White 18
SA49827918_HPPancreas 40-49 Male White 36
SA49828021_HPPancreas 40-49 Male White 5
SA49828119_HPPancreas 40-49 Male White 7
SA49828223_HPPancreas 50-59 Female White 39
SA49828324_HPPancreas 50-59 Male African American 11
SA49828426_HPPancreas 50-59 Male White 10
SA49828525_HPPancreas 50-59 Male White 27
SA49828628_HPPancreas 60-69 Male African American 22
SA49828730_HPPancreas 60-69 Male African American 31
SA49828829_HPPancreas 60-69 Male African American 38
SA49828932_HPPancreas 60-69 Male White 12
SA49829027_HPPancreas 60-69 Male White 25
SA49829131_HPPancreas 60-69 Male White 34
SA49829234_HPPancreas 70-79 Male White 35
SA49829333_HPPancreas 70-79 Male White 9
Showing results 1 to 41 of 41

Collection:

Collection ID:CO004425
Collection Summary:We collected donor pancreata from the Gift of Life Michigan Donor Care Center. The pancreata were either not eligible for transplant or they had no eligible recipients. While maintaining blood flow, we cross-clamped the superior mesenteric and celiac arteries, recovered the pancreas and immediately placed it in a physiologic organ preservation solution on ice. We then transported the pancreata to the University of Michigan, approximately 6 miles away, where it was dissected into head, body and tail, and then immediately flash frozen in liquid nitrogen.
Sample Type:Pancreas

Treatment:

Treatment ID:TR004441
Treatment Summary:These samples are from human donors procured in donation after brain death. Confounding factors, such as donor diseases (e.g., diabetes and obesity), made these organs ineligible for transplantation, which rendered them available for research.

Sample Preparation:

Sampleprep ID:SP004438
Sampleprep Summary:For tissue homogenization, we put tissue samples in 10 μL ice-cold 4:1 LC-grade Methanol:Ultrapure-water per mg tissue and homogenized them using a bead homogenizer. We centrifuged tissue homogenates at 4°C at 21,300 g for 10 minutes and collected the metabolite-containing supernatants. We dried down 100 μL of supernatant from each sample (equivalent to 10 mg of tissue) using a vacuum centrifuge and stored them at -80°C until further processing. Dried supernatants reconstituted in 50ul of LC-grade Methanol: Ultrapure-water mix (1:1).

Chromatography:

Chromatography ID:CH005408
Instrument Name:Agilent 1290 Infinity II
Column Name:Agilent ZORBAX RRHD SB-C18 (150 x 2.1mm,1.8um)
Column Temperature:35
Flow Gradient:0.25 mL/min, 0–2.5 min, 100% A; 7.5 min, 80% A and 20% B; 13 min 55% A and 45% B; 20 min, 1% A and 99% B; 24 min, 1% A and 99% B; 24.05 min, 1% A and 99% C; 27 min, 1% A and 99% C; at 0.8 mL/min, 27.5–31.35 min, 1% A and 99% C; at 0.6 mL/min, 31.50 min, 1% A and 99% C; at 0.4 mL/min, 32.25–39.9 min, 100% A; and at 0.25 mL/min, 40 min, 100% A.
Flow Rate:0.25-0.8 mL/min
Solvent A:97% water/3% methanol;15 mM acetic acid; 10 mM tributylamine at pH 5
Solvent B:100% methanol; 15 mM acetic acid; 10 mM tributylamine
Chromatography Type:Reversed phase
Solvent C:100% acetonitrile, wash solvent.

Analysis:

Analysis ID:AN007118
Laboratory Name:Lyssiotis Lab
Analysis Type:MS
Operator Name:Li Zhang
Chromatography ID:CH005408
Num Factors:41
Num Metabolites:83
Units:AU
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