Summary of Study ST004325

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002739. The data can be accessed directly via it's Project DOI: 10.21228/M8MG1P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST004325
Study TitleDynamics of urine metabolomics and tubular inflammatory cytokines in Type 1 diabetes across disease durations
Study SummaryType 1 diabetes (T1D) is a chronic autoimmune disease characterized by sustained inflammation, leading to diabetic kidney disease (DKD). This study investigated urinary tubular injury biomarkers and metabolomic profiles in relation to albuminuria and renal function across varying durations of T1D. Urinary cytokines (MCP-1, KIM-1, NGAL) were measured by ELISA, and metabolomic profiling was performed using ^1H-NMR spectroscopy. Distinct urinary signatures were identified across T1D duration, including progressive increases in N-acetylcysteine (NAC) and N-delta-acetylornithine (NAO), with decreases in N-acetylaspartate (NAA) and pyruvic acid. These findings suggest early biomarkers of subclinical kidney dysfunction and potential metabolic disturbances associated with T1D progression.
Institute
Lin-Kou Chang Gung Memorial hospital
DepartmentPediatric Nephrology
Last NameYu
First NameMei-Ching
AddressNo. 5, Fuxing St., Guishan Dist., Taoyuan, Taiwan
Emailmc.yu@cgmh.org.tw
Phone+886 3281200 Ext 8202
Submit Date2025-09-29
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2025-11-21
Release Version1
Mei-Ching Yu Mei-Ching Yu
https://dx.doi.org/10.21228/M8MG1P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002739
Project DOI:doi: 10.21228/M8MG1P
Project Title:Dynamics of urine metabolomics and tubular inflammatory cytokines in Type 1 diabetes across disease durations
Project Summary:Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by sustained inflammation, leading to diabetic kidney disease (DKD). This study investigated urinary tubular injury biomarkers and metabolomic profiles in relation to albuminuria and renal function across varying durations of T1D. Urinary cytokines (MCP-1, KIM-1, NGAL) were measured by ELISA, and metabolomic profiling was performed using ^1H-NMR spectroscopy. Distinct urinary signatures were identified across T1D duration, including progressive increases in N-acetylcysteine (NAC) and N-delta-acetylornithine (NAO), with decreases in N-acetylaspartate (NAA) and pyruvic acid. These findings suggest early biomarkers of subclinical kidney dysfunction and potential metabolic disturbances associated with T1D progression.
Institute:Lin-Kou Chang Gung Memorial hospital
Department:Pediatric Nephrology
Last Name:Yu
First Name:Mei-Ching
Address:No. 5, Fuxing St., Guishan Dist., Taoyuan, Taiwan
Email:mc.yu@cgmh.org.tw
Phone:+886 3281200 Ext 8202

Subject:

Subject ID:SU004480
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Duration_group
SA505988DM538Urine T1D-L
SA505989DM400Urine T1D-L
SA505990DM404Urine T1D-L
SA505991DM408Urine T1D-L
SA505992DM452Urine T1D-L
SA505993DM473Urine T1D-L
SA505994DM492Urine T1D-L
SA505995DM495Urine T1D-L
SA505996DM503Urine T1D-L
SA505997DM507Urine T1D-L
SA505998DM511Urine T1D-L
SA505999DM527Urine T1D-L
SA506000DM537Urine T1D-L
SA506001DM541Urine T1D-L
SA506002DM362Urine T1D-L
SA506003DM552Urine T1D-L
SA506004DM555Urine T1D-L
SA506005DM558Urine T1D-L
SA506006DM561Urine T1D-L
SA506007DM005Urine T1D-L
SA506008DM145Urine T1D-L
SA506009DM162Urine T1D-L
SA506010DM215Urine T1D-L
SA506011DM245Urine T1D-L
SA506012DM394Urine T1D-L
SA506013DM398Urine T1D-L
SA506014DM497Urine T1D-L
SA506015DM397Urine T1D-L
SA506016DM348Urine T1D-L
SA506017DM519Urine T1D-L
SA506018DM188Urine T1D-L
SA506019DM002Urine T1D-L
SA506020DM004Urine T1D-L
SA506021DM016Urine T1D-L
SA506022DM103Urine T1D-L
SA506023DM106Urine T1D-L
SA506024DM120Urine T1D-L
SA506025DM135Urine T1D-L
SA506026DM150Urine T1D-L
SA506027DM151Urine T1D-L
SA506028DM159Urine T1D-L
SA506029DM177Urine T1D-L
SA506030DM185Urine T1D-L
SA506031DM203Urine T1D-L
SA506032DM322Urine T1D-L
SA506033DM208Urine T1D-L
SA506034DM221Urine T1D-L
SA506035DM224Urine T1D-L
SA506036DM230Urine T1D-L
SA506037DM241Urine T1D-L
SA506038DM258Urine T1D-L
SA506039DM262Urine T1D-L
SA506040DM310Urine T1D-L
SA506041DM311Urine T1D-L
SA506042DM312Urine T1D-L
SA506043DM317Urine T1D-L
SA506044DM320Urine T1D-L
SA506045DM508Urine T1D-L
SA506046DM013Urine T1D-L
SA506047DM036Urine T1D-L
SA506048DM491Urine T1D-L
SA506049DM336Urine T1D-L
SA506050DM341Urine T1D-L
SA506051DM344Urine T1D-L
SA506052DM352Urine T1D-L
SA506053DM354Urine T1D-L
SA506054DM357Urine T1D-L
SA506055DM372Urine T1D-L
SA506056DM374Urine T1D-L
SA506057DM381Urine T1D-L
SA506058DM418Urine T1D-L
SA506059DM444Urine T1D-L
SA506060DM472Urine T1D-L
SA506061DM496Urine T1D-L
SA506062DM318Urine T1D-L
SA506063DM506Urine T1D-L
SA506064DM529Urine T1D-L
SA506065DM531Urine T1D-L
SA506066DM534Urine T1D-L
SA506067DM543Urine T1D-L
SA506068DM549Urine T1D-L
SA506069DM050Urine T1D-L
SA506070DM087Urine T1D-L
SA506071DM127Urine T1D-L
SA506072DM225Urine T1D-L
SA506073DM264Urine T1D-L
SA506074DM545Urine T1D-L
SA506075DM328Urine T1D-L
SA506076DM298Urine T1D-L
SA506077DM293Urine T1D-L
SA506078DM128Urine T1D-L
SA506079DM044Urine T1D-L
SA506080DM045Urine T1D-L
SA506081DM047Urine T1D-L
SA506082DM058Urine T1D-L
SA506083DM073Urine T1D-L
SA506084DM080Urine T1D-L
SA506085DM081Urine T1D-L
SA506086DM085Urine T1D-L
SA506087DM090Urine T1D-L
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Collection:

Collection ID:CO004473
Collection Summary:Urine samples were collected from youth-onset type 1 diabetes (T1D) patients at Lin-Kou Chang Gung Memorial Hospital, Taiwan, between March 2017 and January 2018. The study aimed to investigate urinary metabolomic profiles and tubular injury biomarkers across different disease durations: ≤5 (S), 6–10 (M), and >10 (L) years.
Sample Type:Urine
Collection Method:Morning voided urine samples were collected and centrifuged at 2000 rpm for 10 min at 4°C. The urinary supernatants were stored at −80°C until NMR analysis.
Collection Location:Department of Pediatric Nephrology, Lin-Kou Chang Gung Memorial Hospital, Taoyuan, Taiwan

Treatment:

Treatment ID:TR004489
Treatment Summary:No treatment or experimental intervention was applied. Participants were classified into groups according to disease duration.

Sample Preparation:

Sampleprep ID:SP004486
Sampleprep Summary:Urine samples (900 μL) were mixed with 100 μL of phosphate buffer prepared in deuterium water (D₂O) containing 0.04% (w/v) TSP (3-trimethylsilyl-propionic-2,2,3,3-d₄ acid sodium salt) for chemical shift calibration. After centrifugation at 12,000 × g for 30 minutes at 4 °C, 600 μL of the supernatant was transferred to NMR tubes for spectral acquisition.

Analysis:

Analysis ID:AN007208
Analysis Type:NMR
Data Format:Bruker FID
Num Factors:3
Num Metabolites:35
Units:relative intensity

NMR:

NMR ID:NM000320
Analysis ID:AN007208
Instrument Name:Bruker Avance 600 MHz NMR Spectrometer
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600 MHz
NMR Solvent:D₂O
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