Summary of Study ST004341

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002753. The data can be accessed directly via it's Project DOI: 10.21228/M8SZ7B This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST004341
Study TitleEffects of honey on alcohol-associated liver disease (ALD): Honey combined with ethanol modeling and serum metabolomics
Study SummaryBackground: Honey, unlike some other common sweeteners, contains diversity of unique biologically active compounds with potential hepato-protective properties. This study aimed to investigate the effects of honey on alcohol-associated liver disease (ALD) and to compare it with a mixture of sugars (39% fructose, 28% glucose, and 4% sucrose). Methods: This study modeled ALD in C57BL/6J mice by feeding them a liquid diet containing 5% ethanol. Concurrently, mice received oral interventions with honey (4 g/kg body weight) and mixed sugars (4 g/kg body weight). At the conclusion of the 18-day experiment, serum, organs, and intestinal contents were collected for analysis. Results: Honey reduced hepatic steatosis, decreased inflammatory gene expression and improved antioxidant capacity compared to mice on a diet mixed with sugar. Meanwhile, honey reduced serum long-chain fatty acid levels and increased short-chain fatty acid (SCFA) levels in cecal contents. In addition, non-targeted metabolomics results indicated that honey ameliorates alcoholic liver injury by modulating steroid hormone biosynthesis and 2 alpha-linolenic acid metabolism. Furthermore, gut flora analyses revealed that honey increased beneficial bacteria such as GCA-900066575 (which produces butyric acid), Dubosiella spp. (which produces propionic acid) and Mucispirillum spp. (which protects the gut barrier). These beneficial bacteria increased SCFA levels in the gut, improved intestinal damage and reduced systemic inflammation. Conclusions: This study demonstrates the potential health benefits of honey in the management of ALD. The mechanisms include remodelling the gut microbiota, promoting metabolic homeostasis and reducing inflammation. The findings o this study provide foundation for the use of honey as a potential dietary intervention in management of metabolic diseases such as ALD.
Institute
Chinese Academy of Agricultural Sciences Institute of Apiculture Research
Last NameZheng
First NameXing
AddressXiangshan, Haidian, Beijing, BEIJING, 100081, China
Emailzhengxing@caas.cn
Phone+86 15600523029
Submit Date2025-11-01
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2025-11-15
Release Version1
Xing Zheng Xing Zheng
https://dx.doi.org/10.21228/M8SZ7B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002753
Project DOI:doi: 10.21228/M8SZ7B
Project Title:Honey combined with ethanol modeling and serum metabolomics
Project Summary:Honey, unlike some other common sweeteners, contains diversity of unique biologically active compounds with potential hepato-protective properties. This study aimed to investigate the effects of honey on alcohol-associated liver disease (ALD) and to compare it with a mixture of sugars (39% fructose, 28% glucose, and 4% sucrose).
Institute:Chinese Academy of Agricultural Sciences Institute of Apiculture Research
Last Name:Zheng
First Name:Xing
Address:Xiangshan, Haidian, Beijing, BEIJING, 100081, China
Email:zhengxing@caas.cn
Phone:+86 15600523029

Subject:

Subject ID:SU004500
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source treatment
SA509388POS_Ethanol group-6Mouse blood Ethanol
SA509389NEG-QC3Mouse blood Ethanol
SA509390NEG-QC4Mouse blood Ethanol
SA509391NEG-QC5Mouse blood Ethanol
SA509392NEG-QC6Mouse blood Ethanol
SA509393NEG_Ethanol group-2Mouse blood Ethanol
SA509394POS_Ethanol group-2Mouse blood Ethanol
SA509395POS_Ethanol group-3Mouse blood Ethanol
SA509396POS_Ethanol group-4Mouse blood Ethanol
SA509397POS_Ethanol group-5Mouse blood Ethanol
SA509398POS_Ethanol group-7Mouse blood Ethanol
SA509399NEG-QC1Mouse blood Ethanol
SA509400POS_Ethanol group-8Mouse blood Ethanol
SA509401POS_Ethanol group-9Mouse blood Ethanol
SA509402POS_Ethanol group-10Mouse blood Ethanol
SA509403POS-QC1Mouse blood Ethanol
SA509404POS-QC2Mouse blood Ethanol
SA509405POS-QC3Mouse blood Ethanol
SA509406POS-QC4Mouse blood Ethanol
SA509407POS-QC5Mouse blood Ethanol
SA509408POS-QC6Mouse blood Ethanol
SA509409NEG-QC2Mouse blood Ethanol
SA509410NEG_Ethanol group-1Mouse blood Ethanol
SA509411POS_Ethanol group-1Mouse blood Ethanol
SA509412NEG_Ethanol group-7Mouse blood Ethanol
SA509413NEG_Ethanol group-10Mouse blood Ethanol
SA509414NEG_Ethanol group-8Mouse blood Ethanol
SA509415NEG_Ethanol group-9Mouse blood Ethanol
SA509416NEG_Ethanol group-6Mouse blood Ethanol
SA509417NEG_Ethanol group-5Mouse blood Ethanol
SA509418NEG_Ethanol group-4Mouse blood Ethanol
SA509419NEG_Ethanol group-3Mouse blood Ethanol
SA509420POS_Ethanol+Honey group-10Mouse blood Ethanol+Honey
SA509421POS_Ethanol+Honey group-8Mouse blood Ethanol+Honey
SA509422POS_Ethanol+Honey group-7Mouse blood Ethanol+Honey
SA509423POS_Ethanol+Honey group-6Mouse blood Ethanol+Honey
SA509424POS_Ethanol+Honey group-5Mouse blood Ethanol+Honey
SA509425POS_Ethanol+Honey group-4Mouse blood Ethanol+Honey
SA509426POS_Ethanol+Honey group-2Mouse blood Ethanol+Honey
SA509427POS_Ethanol+Honey group-1Mouse blood Ethanol+Honey
SA509428POS_Ethanol+Honey group-9Mouse blood Ethanol+Honey
SA509429POS_Ethanol+Honey group-3Mouse blood Ethanol+Honey
SA509430NEG_Ethanol+Honey group-1Mouse blood Ethanol+Honey
SA509431NEG_Ethanol+Honey group-7Mouse blood Ethanol+Honey
SA509432NEG_Ethanol+Honey group-2Mouse blood Ethanol+Honey
SA509433NEG_Ethanol+Honey group-10Mouse blood Ethanol+Honey
SA509434NEG_Ethanol+Honey group-8Mouse blood Ethanol+Honey
SA509435NEG_Ethanol+Honey group-9Mouse blood Ethanol+Honey
SA509436NEG_Ethanol+Honey group-6Mouse blood Ethanol+Honey
SA509437NEG_Ethanol+Honey group-5Mouse blood Ethanol+Honey
SA509438NEG_Ethanol+Honey group-4Mouse blood Ethanol+Honey
SA509439NEG_Ethanol+Honey group-3Mouse blood Ethanol+Honey
SA509440NEG_Ethanol+Sugar group-7Mouse blood Ethanol+Sugar
SA509441POS_Ethanol+Sugar group-8Mouse blood Ethanol+Sugar
SA509442NEG_Ethanol+Sugar group-8Mouse blood Ethanol+Sugar
SA509443NEG_Ethanol+Sugar group-9Mouse blood Ethanol+Sugar
SA509444NEG_Ethanol+Sugar group-10Mouse blood Ethanol+Sugar
SA509445NEG_Ethanol+Sugar group-4Mouse blood Ethanol+Sugar
SA509446NEG_Ethanol+Sugar group-3Mouse blood Ethanol+Sugar
SA509447POS_Ethanol+Sugar group-10Mouse blood Ethanol+Sugar
SA509448POS_Ethanol+Sugar group-9Mouse blood Ethanol+Sugar
SA509449POS_Ethanol+Sugar group-3Mouse blood Ethanol+Sugar
SA509450POS_Ethanol+Sugar group-7Mouse blood Ethanol+Sugar
SA509451POS_Ethanol+Sugar group-6Mouse blood Ethanol+Sugar
SA509452POS_Ethanol+Sugar group-5Mouse blood Ethanol+Sugar
SA509453POS_Ethanol+Sugar group-4Mouse blood Ethanol+Sugar
SA509454POS_Ethanol+Sugar group-2Mouse blood Ethanol+Sugar
SA509455POS_Ethanol+Sugar group-1Mouse blood Ethanol+Sugar
SA509456NEG_Ethanol+Sugar group-2Mouse blood Ethanol+Sugar
SA509457NEG_Ethanol+Sugar group-1Mouse blood Ethanol+Sugar
SA509458NEG_Ethanol+Sugar group-6Mouse blood Ethanol+Sugar
SA509459NEG_Ethanol+Sugar group-5Mouse blood Ethanol+Sugar
Showing results 1 to 72 of 72

Collection:

Collection ID:CO004493
Collection Summary:After the mice inhale carbon dioxide, blood is collected via the orbital sinus, and serum is obtained after centrifugation.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR004509
Treatment Summary:Thirty mice were randomly divided into three groups (n=10/group): (1) Ethanol group (gavage of purified water), (2) Ethanol+Honey group (gavage of 4 g honey /kg body weight), (3) Ethanol+Sugar group (gavage of 4 g mixed sugar/kg body weight).

Sample Preparation:

Sampleprep ID:SP004506
Sampleprep Summary:In brief, serum samples (100 uL) were extracted with 400 uL of methanol and acetonitrile (1:1, v/v), and the extraction efficiency was facilitated by vortexing and sonication. The samples were centrifuged, and the supernatant was dried. The serum was then re-solubilized with methanol and acetonitrile (1:1, v/v). Finally, the supernatant extract was filtered (0.22 um nylon membrane) before metabolite analysis. The instrumentation was based on an Agilent HPLC system combined with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS, 6545, Agilent Technologies, Palo Alto, CA, USA). The test method was also adopted in our previous study.

Combined analysis:

Analysis ID AN007249 AN007250
Chromatography ID CH005502 CH005502
MS ID MS006943 MS006944
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Agilent 1290
Column Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6545 QTOF Agilent 6545 QTOF
Ion Mode NEGATIVE POSITIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH005502
Instrument Name:Agilent 1290
Column Name:Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um)
Column Temperature:30 °C
Flow Gradient:0 min/5% B, 2 min/5% B, 20min/100% B, 25 min/100% B
Flow Rate:0.3 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS006943
Analysis ID:AN007249
Instrument Name:Agilent 6545 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The ion source was ESI, and a nebulizer pressure was 35 psi, a capillary voltage was 3500 V, a fragmentor voltage was 135 V, a drying gas (N2) flow rate was 10 L/min, a drying gas temperature was 325 °C, and a mass range was 100–1700 m/z. The reference MS solution was 112.9856 and 1033.9881to ensure the stability and accuracy of the instrument. NOTE: "Score" (metabolite metadata) is a quantitative index in LC-MS data used to evaluate the reliability of compound matches. A higher Score indicates greater confidence in the compound result.
Ion Mode:NEGATIVE
  
MS ID:MS006944
Analysis ID:AN007250
Instrument Name:Agilent 6545 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The ion source was ESI, and a nebulizer pressure was 35 psi, a capillary voltage was 3500 V, a fragmentor voltage was 135 V, a drying gas (N2) flow rate was 10 L/min, a drying gas temperature was 325 °C, and a mass range was 100–1700 m/z. The reference MS solution was 112.9856 and 1033.9881to ensure the stability and accuracy of the instrument. NOTE: "Score" (metabolite metadata) is a quantitative index in LC-MS data used to evaluate the reliability of compound matches. A higher Score indicates greater confidence in the compound result.
Ion Mode:POSITIVE
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