Summary of Study ST004344

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002756. The data can be accessed directly via it's Project DOI: 10.21228/M8DR9M This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST004344
Study TitleThe chemical profiles of Aroeira honey from Vale do Jequitinhonha and from Norte de Minas (Brazil) are largely similar
Study Typeuntargeted metabolomics
Study SummaryThe aroeira tree (Myracrodruon urundeuva) grows in the semi-arid region of Minas Gerais, bearing flowers that yield a special honey with recognized therapeutic properties. The objective of this study was to compare the chemical profile of aroeira honey from the Vale do Jequitinhonha with the one of aroeira honey produced in the Norte de Minas region. The chemical composition of the samples was analyzed by an untargeted metabolomic approach using liquid chromatography-mass spectrometry. Despite few specific compounds, the aroeira honeys from these two mesoregions (Vale do Jequitinhonha and Norte de Minas) could not be differentiated based on their chemical profiles. We conclude that it is appropriate to consider aroeira honeys as chemically similar throughout the semi-arid region of Minas Gerais.
Institute
Federal University of Minas Gerais
DepartmentDepartment of Chemistry - UFMG
LaboratoryLaboratory of Analytical Chemistry and Mass Spectrometry
Last NameNori de Macedo
First NameAdriana
AddressAv. Antônio Carlos, 6627
Emailadrianandm@gmail.com
Phone+553134095756
Submit Date2025-08-14
Num Groups4
Total Subjects48
PublicationsSilva, M. C. M. et al. Chemical fingerprints of the organic varietal aroeira honey supports Geographical Indication area expansion in Minas Gerais, Brazil. J. Apic. Res. https://doi.org/10.1080/00218839.2024.2430850
Raw Data AvailableYes
Raw Data File Type(s)d, mzXML
Analysis Type DetailLC-MS
Release Date2025-12-05
Release Version1
Adriana Nori de Macedo Adriana Nori de Macedo
https://dx.doi.org/10.21228/M8DR9M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002756
Project DOI:doi: 10.21228/M8DR9M
Project Title:Chemical fingerprint of aroeira honey to support Geographical Indication area expansion in Minas Gerais, Brazil
Project Type:MS-based untargeted metabolomics study
Project Summary:The aroeira tree (Myracrodruon urundeuva) grows in the semi-arid region of Minas Gerais, bearing flowers that yield a special honey with recognized therapeutic properties. The objective of this study was to compare the chemical profile of aroeira honey from the Vale do Jequitinhonha with the one of aroeira honey produced in the Norte de Minas region. The chemical composition of the samples was analyzed by an untargeted metabolomic approach using liquid chromatography-mass spectrometry. Despite few specific compounds, the aroeira honeys from these two mesoregions (Vale do Jequitinhonha and Norte de Minas) could not be differentiated based on their chemical profiles. We conclude that it is appropriate to consider aroeira honeys as chemically similar throughout the semi-arid region of Minas Gerais.
Institute:Federal University of Minas Gerais
Department:Department of Chemistry
Laboratory:Laboratory of Analytical Chemistry and Mass Spectrometry
Last Name:Nori de Macedo
First Name:Adriana
Address:Av. Antonio Carlos, 6627, Belo Horizonte, Minas Gerais, 31270901, Brazil
Email:adrianandm@gmail.com
Phone:+55-31-34095756
Funding Source:FAPEMIG, CNPq, CAPES, MCT/FINEP/CT-INFRA
Publications:Silva, M. C. M. et al. Chemical fingerprints of the organic varietal aroeira honey supports Geographical Indication area expansion in Minas Gerais, Brazil. J. Apic. Res., 64(5), 1352-1361, https://doi.org/10.1080/00218839.2024.2430850
Contributors:Mayra Conceicao Meira Silva, Adriana Nori de Macedo, Olivia Brito de Oliveira Moreira, Marcone Augusto Leal de Oliveira, Zong-Xin Ren, Andre Rodrigo Rech

Subject:

Subject ID:SU004503
Subject Type:Food item

Factors:

Subject type: Food item; Subject species: - (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Origin
SA515987JC40Aroeira Honey Jequi-Coop
SA515988JC20Aroeira Honey Jequi-Coop
SA515989JC19Aroeira Honey Jequi-Coop
SA515990JC18Aroeira Honey Jequi-Coop
SA515991JC15Aroeira Honey Jequi-Coop
SA515992JC14Aroeira Honey Jequi-Coop
SA515993JC12Aroeira Honey Jequi-Coop
SA515994JC10Aroeira Honey Jequi-Coop
SA515995JC09Aroeira Honey Jequi-Coop
SA515996JC08Aroeira Honey Jequi-Coop
SA515997JC04Aroeira Honey Jequi-Coop
SA515998JC01Aroeira Honey Jequi-Coop
SA515999JC17Aroeira Honey Jequi-Coop
SA516000JI63Aroeira Honey Jequi-Isolated
SA516001JI73Aroeira Honey Jequi-Isolated
SA516002JI71Aroeira Honey Jequi-Isolated
SA516003JI69Aroeira Honey Jequi-Isolated
SA516004JI68Aroeira Honey Jequi-Isolated
SA516005JI67Aroeira Honey Jequi-Isolated
SA516006JI66Aroeira Honey Jequi-Isolated
SA516007JI65Aroeira Honey Jequi-Isolated
SA516008JI64Aroeira Honey Jequi-Isolated
SA516009JI62Aroeira Honey Jequi-Isolated
SA516010JI61.1Aroeira Honey Jequi-Isolated
SA516011JI61Aroeira Honey Jequi-Isolated
SA516012N44Aroeira Honey North
SA516013N56Aroeira Honey North
SA516014N43Aroeira Honey North
SA516015N55Aroeira Honey North
SA516016N45Aroeira Honey North
SA516017N53Aroeira Honey North
SA516018N42Aroeira Honey North
SA516019N58Aroeira Honey North
SA516020N59Aroeira Honey North
SA516021N60Aroeira Honey North
SA516022N54Aroeira Honey North
SA516023N57Aroeira Honey North
SA516024N52Aroeira Honey North
SA516025N47Aroeira Honey North
SA516026N51Aroeira Honey North
SA516027N46Aroeira Honey North
SA516028N41Aroeira Honey North
SA516029N48Aroeira Honey North
SA516030N49Aroeira Honey North
SA516031N50Aroeira Honey North
SA516032QC2PooledSample QC
SA516033QC3PooledSample QC
SA516034QC4PooledSample QC
SA516035QC5PooledSample QC
SA516036QC6PooledSample QC
SA516037QC7PooledSample QC
SA516038QC8PooledSample QC
SA516039QC9PooledSample QC
SA516040QC10PooledSample QC
SA516041QC1PooledSample QC
SA516042W25Wild Honey Jequi-Wild
SA516043W26Wild Honey Jequi-Wild
SA516044W29Wild Honey Jequi-Wild
Showing results 1 to 58 of 58

Collection:

Collection ID:CO004496
Collection Summary:In Vale do Jequitinhonha, samples of aroeira honey were obtained from 13 apiaries of beekeepers who were members of the Cooperative of Beekeepers of the Vale do Jequitinhonha (COOAPIVAJE), identified as “Jequi-Coop”, and 12 samples of aroeira honey from beekeepers who were not part of the Cooperative, herein called isolated beekeepers (“Jequi-Isolated”). Samples were collected from May to August 2021, when the aroeira tree is in bloom. The 20 samples from Norte de Minas (“North”) were kindly provided by the Cooperative of Beekeepers and Family Farmers of Norte de Minas (COOPEMAPI). A few (three) samples of wild honey from Vale do Jequitinhonha were also included as a type of negative control (non-aroeira honey).
Sample Type:Honey
Storage Conditions:Room temperature
Additives:None

Treatment:

Treatment ID:TR004512
Treatment Summary:No other treatments were performed to the samples prior do sample preparation.

Sample Preparation:

Sampleprep ID:SP004509
Sampleprep Summary:About 0.2 g of each honey sample was transferred to 2 mL microcentrifuge (actual masses recorded for posterior data normalization). A 500 µL aliquot of methanol:acetonitrile at a 1:1 (v/v) ratio was added to each microtube, along with 10 µL of 200 mg/L F-Phe solution (internal standard). The mixtures were homogenized by vortex for 10 s and sonicated on ice for 20 min. Samples were then centrifuged at 7000 rpm for 5 min at room temperature (≈ 25 °C) to facilitate the collection of the supernatant extract, which was transferred to a chromatographic vial, diluted 4-fold in LC-MS grade water and stored at -18 °C until instrumental analysis.

Combined analysis:

Analysis ID AN007253 AN007254
Chromatography ID CH005505 CH005505
MS ID MS006947 MS006948
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1260 Agilent 1260
Column Phenomenex Kinetex PFP (150 x 4.6 mm, 5um) Phenomenex Kinetex PFP (150 x 4.6 mm, 5um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH005505
Instrument Name:Agilent 1260
Column Name:Phenomenex Kinetex PFP (150 x 4.6 mm, 5um)
Column Temperature:40
Flow Gradient:0.0 min to 12.0 min from 1% B to 95% B, 12.0 min to 15.0 min maintained at 95% B, 15.0 min to 16.0 min from 95% B to 1% B, 16.0 min to 20.0 min maintained at 1% B for column equilibration.
Flow Rate:0.6 mL/min
Internal Standard:Fluorophenylalanine
Sample Injection:5 µL
Solvent A:100% Water; 0.5% formic acid
Solvent B:100% Methanol; 0.5% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS006947
Analysis ID:AN007253
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Mass spectrometer conditions were: acquisition in scan mode from 100 to 3000 m/z (2 spectra/s), nebulizer gas temperature: 350 °C, gas flow: 12 L/min, nebulizer pressure: 50 psig, sheath gas temperature: 325 °C, sheath gas flow: 10 L/min, capillary voltage: 3500 V, fragmentor voltage: 125 V, skimmer voltage: 47 V, and octopole: 750 V. Purine, ammonium trifluoroacetate, and HP-0921 (API-TOF Reference Mass Solution Kit, Agilent Technologies) were added to the mobile phase as reference masses for real-time automatic mass calibration, providing ions in positive ion mode with m/z 121.0509 (protonated purine) and m/z 922.0098 (protonated HP-0921), as well as in negative ion mode with m/z 112.9856 (trifluoroacetate) and m/z 966.0007 (formate adduct of HP-0921). Acquisition was performed separately in positive and negative ionization modes in high resolution (HiRes 4 GHz) and data was stored in a centroid format.
Ion Mode:POSITIVE
Capillary Voltage:3500
Dry Gas Flow:12 L/min
Fragment Voltage:125
Ionization:Positive
Octpole Voltage:750
  
MS ID:MS006948
Analysis ID:AN007254
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Mass spectrometer conditions were: acquisition in scan mode from 100 to 3000 m/z (2 spectra/s), nebulizer gas temperature: 350 °C, gas flow: 12 L/min, nebulizer pressure: 50 psig, sheath gas temperature: 325 °C, sheath gas flow: 10 L/min, capillary voltage: 3500 V, fragmentor voltage: 125 V, skimmer voltage: 47 V, and octopole: 750 V. Purine, ammonium trifluoroacetate, and HP-0921 (API-TOF Reference Mass Solution Kit, Agilent Technologies) were added to the mobile phase as reference masses for real-time automatic mass calibration, providing ions in positive ion mode with m/z 121.0509 (protonated purine) and m/z 922.0098 (protonated HP-0921), as well as in negative ion mode with m/z 112.9856 (trifluoroacetate) and m/z 966.0007 (formate adduct of HP-0921). Acquisition was performed separately in positive and negative ionization modes in high resolution (HiRes 4 GHz) and data was stored in a centroid format.
Ion Mode:NEGATIVE
Capillary Voltage:3500
Dry Gas Flow:12 L/min
Fragment Voltage:125
Ionization:Negative
Octpole Voltage:750
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