Summary of Study ST004388
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002781. The data can be accessed directly via it's Project DOI: 10.21228/M8626Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004388 |
| Study Title | Untargeted metabolomics to unravel the adaptations of the desert shrub Haloxylon salicornicum |
| Study Summary | This study investigated the metabolic adjustments of the desert shrub Haloxylon salicornicum during drought and subsequent recovery after rewatering. An untargeted metabolomics approach using Orbitrap GC–MS was employed to profile the primary metabolites accumulated in shoots and roots in response to 15 days of water withholding followed by 15 days of rewatering. Six-month-old H. salicornicum seedlings grown in pots were divided into two groups: control plants irrigated every other day and drought-stressed plants subjected to water withholding for 15 days. Shoots and roots were sampled from four seedlings in each group, while the remaining seedlings from both control and drought-stressed treatments were rewatered for an additional 15 days for recovery assessment. Comparisons of the metabolic profiles were made between control and drought-stressed seedlings after 15 days of treatment, and between rewatered and control seedlings after 15 days of recovery. Drought exposure induced the accumulation of 25 metabolites in shoots and 24 in roots, among which eight compounds, γ-aminobutyrate, glutathione, cystine, valine, leucine, ribitol, succinate, and N-acetylputrescine were consistently upregulated in both organs. These shared metabolites collectively represent an integrated drought-response strategy involving stress signal transduction, antioxidant defense, and osmotic adjustment to sustain cellular homeostasis under drought. Shoots specifically accumulated metabolites such as oleic acid and galacturonate, which are implicated in cuticle biosynthesis and cell wall remodeling, as well as amino acids and tricarboxylic acids supporting energy supply and C-N balance under drought. In contrast, roots exhibited elevated levels of sugar alcohols contributing to osmotic adjustment, along with increased accumulation of UDP-glucuronate, which reinforces cell wall rigidity. Following rehydration, seedlings did not fully return to the pre-stress metabolic state but displayed partial recovery characterized by renewed carbohydrate metabolism and sustained levels of stress-related metabolites. |
| Institute | Sultan Moulay Slimane University |
| Last Name | Ezzine |
| First Name | Hasna |
| Address | Bzou, Azilal, Morocco |
| hasnaezzine1@gmail.com | |
| Phone | +212648297165 |
| Submit Date | 2025-11-13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Thermo) |
| Analysis Type Detail | GC-MS |
| Release Date | 2025-12-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002781 |
| Project DOI: | doi: 10.21228/M8626Q |
| Project Title: | Desert plant metabolomics |
| Project Type: | Untargeted metabolomics |
| Project Summary: | This project aims to identify the primary metabolites involved in desert plant adaptations to desert harshness |
| Institute: | Sultan Moulay Slimane University |
| Department: | Biology & Geology department |
| Laboratory: | Polyvalent team in Research & development |
| Last Name: | Ezzine |
| First Name: | Hasna |
| Address: | Bzou, Azilal, Morocco |
| Email: | hasnaezzine1@gmail.com |
| Phone: | +212648297165 |
Subject:
| Subject ID: | SU004547 |
| Subject Type: | Plant |
| Subject Species: | Haloxylon salicornicum |
| Taxonomy ID: | 454511 |
Factors:
Subject type: Plant; Subject species: Haloxylon salicornicum (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Treatment |
|---|---|---|---|
| SA520983 | CR4 | Root | Control 15D |
| SA520984 | CR1 | Root | Control 15D |
| SA520985 | CR2 | Root | Control 15D |
| SA520986 | CR3 | Root | Control 15D |
| SA520987 | CMR3 | Root | Control 1M |
| SA520988 | CMR4 | Root | Control 1M |
| SA520989 | CMR2 | Root | Control 1M |
| SA520990 | CMR1 | Root | Control 1M |
| SA520991 | DR1 | Root | Drought |
| SA520992 | DR2 | Root | Drought |
| SA520993 | DR3 | Root | Drought |
| SA520994 | DR4 | Root | Drought |
| SA520995 | RR1 | Root | Recovery |
| SA520996 | RR3 | Root | Recovery |
| SA520997 | RR4 | Root | Recovery |
| SA520998 | RR2 | Root | Recovery |
| SA520999 | CS1 | Shoot | Control 15D |
| SA521000 | CS4 | Shoot | Control 15D |
| SA521001 | CS2 | Shoot | Control 15D |
| SA521002 | CS3 | Shoot | Control 15D |
| SA521003 | CMS3 | Shoot | Control 1M |
| SA521004 | CMS4 | Shoot | Control 1M |
| SA521005 | CMS2 | Shoot | Control 1M |
| SA521006 | CMS1 | Shoot | Control 1M |
| SA521007 | DS4 | Shoot | Drought |
| SA521008 | DS3 | Shoot | Drought |
| SA521009 | DS2 | Shoot | Drought |
| SA521010 | DS1 | Shoot | Drought |
| SA521011 | RS1 | Shoot | Recovery |
| SA521012 | RS2 | Shoot | Recovery |
| SA521013 | RS3 | Shoot | Recovery |
| SA521014 | RS4 | Shoot | Recovery |
| Showing results 1 to 32 of 32 |
Collection:
| Collection ID: | CO004540 |
| Collection Summary: | Shoot and root tissues were immediately frozen in liquid nitrogen during sampling befoe being freeze-dried and powdered |
| Sample Type: | plant tissue |
Treatment:
| Treatment ID: | TR004556 |
| Treatment Summary: | Haloxylon salicornicum seedlings were grown in pots containing 75% of sand and 25% of peat moss. The seedlings were divided into two groups: control plants irrigated every other day and drought-stressed plants subjected to water withholding for 15 days. Shoots and roots were sampled from four seedlings in each group, while the remaining seedlings from both control and drought-stressed treatments were rewatered for an additional 15 days for recovery assessment. |
Sample Preparation:
| Sampleprep ID: | SP004553 |
| Sampleprep Summary: | Approximately 30 mg of the powdered tissue was transferred to 2 mL Eppendorf tubes containing a few 0.5 mm glass beads, and 1 mL of M1 (methyl-tert-bethyl ether/ methanol; 3:1) was added. The mixture was then homogenized in the Bead Blaster 24R (Benchmark Scientific) by subjecting it to 3 cycles of shaking at 4 °C for 1.5 minutes, followed by an immediate sonication at 0 °C for 20 min. A 650 µL of the phase separation solution M2 (water/methanol; 3:1) was added, and the homogenate was immediately vortexed for 1 min and then centrifuged at 20,000 rpm for 2 min. |
Combined analysis:
| Analysis ID | AN007331 |
|---|---|
| Chromatography ID | CH005563 |
| MS ID | MS007025 |
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Thermo Scientific Exploris GC 240 |
| Column | Thermo Scientific Trace GOLD TG-5SilMS (30 m × 0.25 mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Orbitrap Exploris 240 |
| Ion Mode | POSITIVE |
| Units | peak intensity |
Chromatography:
| Chromatography ID: | CH005563 |
| Instrument Name: | Thermo Scientific Exploris GC 240 |
| Column Name: | Thermo Scientific Trace GOLD TG-5SilMS (30 m × 0.25 mm, 0.25 um) |
| Column Temperature: | 250 °C |
| Flow Gradient: | - |
| Flow Rate: | 1.2 mL/min |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | GC |
MS:
| MS ID: | MS007025 |
| Analysis ID: | AN007331 |
| Instrument Name: | Thermo Orbitrap Exploris 240 |
| Instrument Type: | Orbitrap |
| MS Type: | EI |
| MS Comments: | The mass spectrometer was operated in positive electron ion mode within a mass-to-charge (m/z) scan range of 35-700, at a temperature of 300 °C, with an orbitrap resolution of 60,000 (FWHM, measured at m/z 200) |
| Ion Mode: | POSITIVE |
