Summary of Study ST004454
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002814. The data can be accessed directly via it's Project DOI: 10.21228/M8XG1G This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST004454 |
| Study Title | Untargeted Metabolomics Reveals Divergent Metabolic Profiles Between the Predatory Arma chinensis and the Phytophagous Halyomorpha halys |
| Study Summary | Arma chinensis, a predatory insect, and Halyomorpha halys, a phytophagous insect, exhibit a predator–prey relationship, which poses a distinct physiological challenge. Although the dietary contrast between the two bugs provides an ideal model system for investigating insect metabolic adaptation mechanisms, previous research has not elucidated this. We aimed to clarify the metabolic adaptations associated with different feeding habits of A. chinensis and H. halys. Untargeted metabolomics was used to compare the global metabolic profiles of these insects. |
| Institute | Institute of Plant Protection, Chinese Academy of Agricultural Sciences |
| Last Name | Liu |
| First Name | Chenxi |
| Address | No. 2 Yuanmingyuan West Road, Beijing, Haidian District, 100193, China |
| liuchenxi@caas.cn | |
| Phone | 13269116665 |
| Submit Date | 2025-11-26 |
| Num Groups | 4 |
| Total Subjects | 24 |
| Num Males | 12 |
| Num Females | 12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2026-01-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002814 |
| Project DOI: | doi: 10.21228/M8XG1G |
| Project Title: | Untargeted Metabolomics Reveals Divergent Metabolic Profiles Between the Predatory Arma chinensis and the Phytophagous Halyomorpha halys |
| Project Summary: | Arma chinensis, a predatory insect, and Halyomorpha halys, a phytophagous insect, exhibit a predator–prey relationship, which poses a distinct physiological challenge. Although the dietary contrast between the two bugs provides an ideal model system for investigating insect metabolic adaptation mechanisms, previous research has not elucidated this. We aimed to clarify the metabolic adaptations associated with different feeding habits of A. chinensis and H. halys. Untargeted metabolomics was used to compare the global metabolic profiles of these insects. |
| Institute: | Institute of Plant Protection, Chinese Academy of Agricultural Sciences |
| Last Name: | Liu |
| First Name: | Chenxi |
| Address: | No. 2 Yuanmingyuan West Road, Beijing, Haidian District, 100193, China |
| Email: | liuchenxi@caas.cn |
| Phone: | 13269116665 |
Subject:
| Subject ID: | SU004616 |
| Subject Type: | Insect |
| Subject Species: | Arma chinensis, Halyomorpha halys |
| Taxonomy ID: | 763200, 286706 |
| Age Or Age Range: | adult |
| Weight Or Weight Range: | 80-100 mg |
| Height Or Height Range: | 7.8–10.2 mm |
| Gender: | Male and female |
Factors:
Subject type: Insect; Subject species: Arma chinensis, Halyomorpha halys (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Species | Sex |
|---|---|---|---|---|
| SA527016 | Arma_F6 | insect | Arma chinensis | F |
| SA527017 | Arma_F5 | insect | Arma chinensis | F |
| SA527018 | Arma_F4 | insect | Arma chinensis | F |
| SA527019 | Arma_F3 | insect | Arma chinensis | F |
| SA527020 | Arma_F2 | insect | Arma chinensis | F |
| SA527021 | Arma_F1 | insect | Arma chinensis | F |
| SA527022 | Arma_M2 | insect | Arma chinensis | M |
| SA527023 | Arma_M6 | insect | Arma chinensis | M |
| SA527024 | Arma_M5 | insect | Arma chinensis | M |
| SA527025 | Arma_M4 | insect | Arma chinensis | M |
| SA527026 | Arma_M3 | insect | Arma chinensis | M |
| SA527027 | Arma_M1 | insect | Arma chinensis | M |
| SA527028 | Haly_F6 | insect | Halyomorpha halys | F |
| SA527029 | Haly_F5 | insect | Halyomorpha halys | F |
| SA527030 | Haly_F4 | insect | Halyomorpha halys | F |
| SA527031 | Haly_F3 | insect | Halyomorpha halys | F |
| SA527032 | Haly_F2 | insect | Halyomorpha halys | F |
| SA527033 | Haly_F1 | insect | Halyomorpha halys | F |
| SA527034 | Haly_M2 | insect | Halyomorpha halys | M |
| SA527035 | Haly_M6 | insect | Halyomorpha halys | M |
| SA527036 | Haly_M5 | insect | Halyomorpha halys | M |
| SA527037 | Haly_M4 | insect | Halyomorpha halys | M |
| SA527038 | Haly_M3 | insect | Halyomorpha halys | M |
| SA527039 | Haly_M1 | insect | Halyomorpha halys | M |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO004609 |
| Collection Summary: | The initial A. chinensis population was collected from the field in Beijing, China. Successive generations were reared in the laboratory on an artificial prey diet containing Antheraea pernyi pupae until a stable colony was established. Larvae were housed in rearing containers (8.5 cm diameter × 10 cm height) with mesh lids. Absorbent cotton moistened with water was formed into balls and placed on top of the mesh lid to supply drinking water to A. chinensis. Fresh A. pernyi pupae were provided every three days as food. The rearing containers were maintained in a climate-controlled incubator set at 26 ± 1°C, 65% ± 5% relative humidity, and a 16L:8D photoperiod. A. pernyi pupae used to feed A. chinensis were stored at 4°C to preserve their condition. Adult H. halys individuals were collected from Sanming City, Fujian Province, China. An experimental colony was established and maintained in a rearing container (32 cm × 20 cm × 18 cm). Fresh corn was provided as the food source, while moistened absorbent cotton balls supplied water. Food was replaced, moisture was replenished, and eggs were removed every 3 days. The rearing containers were maintained in a climate-controlled incubator at 26 ± 1°C, 65% ± 5% relative humidity, and a 16L:8D photoperiod. Corn used for feeding H. halys was stored at 4°C to maintain its freshness. Newly emerged adult females and males of A. chinensis and H. halys were selected for analysis. Before experimentation, all specimens were acclimated under uniform laboratory incubator conditions (26 ± 1°C, 65% ± 5% relative humidity, and 16L:8D photoperiod) for 2 weeks. |
| Sample Type: | Insect tissue |
| Collection Method: | liquid nitrogen quick freezing |
| Collection Location: | Beijing, China. |
| Collection Duration: | 1 day |
| Volumeoramount Collected: | 240 |
| Storage Conditions: | -80℃ |
| Collection Vials: | rearing containers (8.5 cm diameter × 10 cm height) |
| Storage Vials: | rearing containers (8.5 cm diameter × 10 cm height) |
| Collection Tube Temp: | liquid nitrogen quick freezing |
| Additives: | without |
Treatment:
| Treatment ID: | TR004625 |
| Treatment Summary: | Newly emerged adults of both species, after 2 weeks of acclimation, were collected and divided into four groups: (1) A. chinensis females (AF), (2) A. chinensis males (AM), (3) H. halys females (HF), and (4) H. halys males (HM). The samples were surface-cleaned with 75% ethanol, rinsed twice with distilled water, flash-frozen in liquid nitrogen, and stored at -80°C until analysis. Metabolomic sequencing was performed by Shanghai Personalbio Technology Co., Ltd. |
| Treatment: | No processing |
| Treatment Compound: | No processing |
| Treatment Route: | No processing |
| Treatment Dose: | No processing |
| Treatment Dosevolume: | No processing |
| Treatment Doseduration: | No processing |
| Treatment Vehicle: | No processing |
Sample Preparation:
| Sampleprep ID: | SP004622 |
| Sampleprep Summary: | Newly emerged adults of both species, after 2 weeks of acclimation, were collected and divided into four groups: (1) A. chinensis females (AF), (2) A. chinensis males (AM), (3) H. halys females (HF), and (4) H. halys males (HM). The samples were surface-cleaned with 75% ethanol, rinsed twice with distilled water, flash-frozen in liquid nitrogen, and stored at -80°C until analysis. Metabolomic sequencing was performed by Shanghai Personalbio Technology Co., Ltd.Metabolite Extraction Procedure: 1.Sample Preparation: Precisely weigh 30 mg of the sample and place it into a 2 mL centrifuge tube. 2.Grinding: Add 200 μL of pre-chilled water and two steel beads. Grind the sample using a high-throughput tissue grinder (55 Hz) for 60 seconds. Repeat this grinding step once. 3.Solvent Extraction: Add 800 μL of pre-chilled methanol:acetonitrile mixed solution (1:1, v/v). 4.Sonication and Freezing: Place the centrifuge tube in an ultrasonic cleaner and sonicate for 30 minutes. Subsequently, transfer the tube to a -20°C freezer and incubate for 45 minutes. 5.Centrifugation and Concentration: Centrifuge the sample at 12,000 rpm for 20 minutes at 4°C. Carefully pipette 800 μL of the supernatant. Vacuum-concentrate the supernatant until completely dry. 6.Reconstitution: Add 150 μL of a 50% methanol solution containing 5 ppm 2-chloro-L-phenylalanine to reconstitute the dried extract. Vortex-mix for 30 seconds. 7.Cleanup and Loading: Centrifuge the solution at 12,000 rpm for 10 minutes at 4°C. Transfer the supernatant and filter it through a 0.22 μm membrane filter. Centrifuge the filtrate again at 12,000 rpm for 10 minutes at 4°C. Transfer the resulting supernatant to an injection vial for analysis. 8.Quality Control (QC) Sample Preparation: When the number of samples exceeds 5, pipette 10-20 μL from the filtered solution of each individual sample, pool them together, and mix thoroughly to prepare one QC sample. This QC sample is used to assess instrument stability and data reliability. QC sample preparation is not required when the number of samples is ≤5. |
| Processing Method: | Separate the newly emerged female and male adult insects, and rear them in the laboratory incubator under the same conditions (temperature 26 ± 1℃, relative humidity 65% ± 5%, photoperiod 16L:8D) for two weeks for the experiment. |
| Processing Storage Conditions: | Room temperature |
| Extraction Method: | Weigh 30 mg of the sample in a 2 mL centrifuge tube, add 200 μL of pre-cooled water, and 2 steel beads. Put it into the high-throughput tissue homogenizer and homogenize at 55 Hz for 60 seconds. Repeat this step once; add 800 μL of methanol:acetonitrile (1:1, v/v); put it in the ultrasonic cleaning machine for ultrasonic treatment for 30 minutes; place it in the -20℃ refrigerator for freezing for 45 minutes; centrifuge at 12,000 rpm and 4℃ for 20 minutes, take the supernatant 800 μL, vacuum concentrate until dry; add 150 μL of 50% methanol (containing 5 ppm 2-chloropropylalanine) to dissolve, vortex for 30 seconds; centrifuge at 12,000 rpm and 4℃ for 10 minutes, take the supernatant, pass through a 0.22 μm filter membrane, and centrifuge again at 12,000 rpm and 4℃ for 10 minutes, and add it to the analysis bottle for analysis. |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN007458 | AN007459 |
|---|---|---|
| Chromatography ID | CH005648 | CH005648 |
| MS ID | MS007150 | MS007151 |
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish Flex | Thermo Vanquish Flex |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1 mm, 1.8 μm, 100 Å) | Waters ACQUITY UPLC HSS T3 (100 x 2.1 mm, 1.8 μm, 100 Å) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Orbitrap Exploris 120 | Thermo Orbitrap Exploris 120 |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak intensity | Peak intensity |
Chromatography:
| Chromatography ID: | CH005648 |
| Chromatography Summary: | The ACQUITY UPLC HSS T3 (100 Å, 1.8 μm, 2.1 mm × 100 mm) column was used, with column temperature: 40°C; autosampler temperature: 8°C; and injection volume: 2 μL. The flow rate was set at 0.4 mL/min. The mobile phase consisted of 0.1% formic acid in water (A) and acetonitrile (containing 0.1% formic acid; B). |
| Instrument Name: | Thermo Vanquish Flex |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1 mm, 1.8 μm, 100 Å) |
| Column Temperature: | 40℃ |
| Flow Gradient: | The percentages below refer to the solvent B%. The elution gradient starts at 5% and lasts for 1 minute. Then, it linearly increases to 95% within 1 to 4.7 minutes, and remains at 95% for 1.3 minutes until the 6th minute, followed by a rapid drop to the initial 5% within 0.1 minutes (from 6.0 to 6.1 minutes), and finally stays at 5% for 2.4 minutes until the total run time of 8.5 minutes ends. |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% Acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS007150 |
| Analysis ID: | AN007458 |
| Instrument Name: | Thermo Orbitrap Exploris 120 |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The Thermo Orbitrap Exploris 120 mass spectrometer was used to collect DDA mass spectrometric data in the positive and negative ion modes using Xcalibur software (version 4.7; Thermo). For HESI source, the following settings were used: spray voltage: 3.5/-3.0 kV, sheath gas: 40 arb, auxiliary gas 15 arb, capillary temperature 325℃, auxiliary gas temperature 300℃, primary resolution: 60,000, scan range: 100–1000 m/z, AGC Target: Standard, and Max IT: 100 ms. The top 4 ions were screened for secondary fragmentation, with dynamic exclusion time: 8 s, secondary resolution: 15,000, HCD collision energy: 30%, AGC Target: Standard, and Max IT: Auto. |
| Ion Mode: | POSITIVE |
| MS ID: | MS007151 |
| Analysis ID: | AN007459 |
| Instrument Name: | Thermo Orbitrap Exploris 120 |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The Thermo Orbitrap Exploris 120 mass spectrometer was used to collect DDA mass spectrometric data in the positive and negative ion modes using Xcalibur software (version 4.7; Thermo). For HESI source, the following settings were used: spray voltage: 3.5/-3.0 kV, sheath gas: 40 arb, auxiliary gas 15 arb, capillary temperature 325℃, auxiliary gas temperature 300℃, primary resolution: 60,000, scan range: 100–1000 m/z, AGC Target: Standard, and Max IT: 100 ms. The top 4 ions were screened for secondary fragmentation, with dynamic exclusion time: 8 s, secondary resolution: 15,000, HCD collision energy: 30%, AGC Target: Standard, and Max IT: Auto. |
| Ion Mode: | NEGATIVE |
