Summary of Study ST004527

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002853. The data can be accessed directly via it's Project DOI: 10.21228/M8W847 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST004527
Study Title	Flavonoids-rich silage reprogram rumen microbiota and flavonoid metabolic profile.
Study Type	Research
Study Summary	To study the specific mechanism through which flavonoid-rich silage modulates rumen microbiota composition and flavonoid metabolites, two groups of dairy goats were fed with different silage feeds to investigate their ruminal flavonoid metabolic profile. Results showed that feeding flavonoid-rich BX62 silage increase the flavonoid metabolites and flavonoid-metabolizing microorganisms in the rumen. These results demonstrate that silage-derived flavonoids drive rumen microbiome remodeling and flavonoid metabolic activity.
Institute	Lanzhou University
Department	School of Life Sciences
Laboratory	State Key Laboratory of Grassland and Agro-ecosystems
Last Name	Ma
First Name	Jing
Address	No. 222, Tianshui South Road, Lanzhou, Gansu, 730000, China
Email	mjing2023@lzu.edu.cn
Phone	+86 19852806670
Submit Date	2026-01-03
Raw Data Available	Yes
Raw Data File Type(s)	mzML, raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2026-01-15
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR002853
Project DOI:	doi: 10.21228/M8W847
Project Title:	Silage-derived flavonoids can reprogram rumen microbiota and metabolic profile.
Project Type:	Research
Project Summary:	Feeding LAB-inoculated silage modulates rumen microbiota composition and metabolites, thereby improving ruminant production performance. The goal of the project is to explore how LAB inoculants affect the rumen metabolic profile. Results showed that inoculation with homofermentative Lactiplantibacillus plantarum BX62 improved the alfalfa flavonoid content. Feeding flavonoid-rich BX62 silage reshaped the rumen microbiota, which resulted in the increase in flavonoid metabolites, the growth of flavonoid-metabolizing microorganisms, and the upregulation of the flavonoid and carbohydrate metabolic pathways in the rumen. These results demonstrate that silage-derived flavonoids can reprogram rumen microbiota and metabolic profile.
Institute:	Lanzhou University
Department:	School of Life Sciences
Laboratory:	State Key Laboratory of Grassland and Agro-ecosystems
Last Name:	Ma
First Name:	Jing
Address:	No. 222, Tianshui South Road, Lanzhou, Gansu, 730000, China
Email:	mjing2023@lzu.edu.cn
Phone:	+86 19852806670

Subject:

Subject ID:	SU004706
Subject Type:	Mammal
Subject Species:	Capra hircus
Taxonomy ID:	9925

Factors:

Subject type: Mammal; Subject species: Capra hircus (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample source	Feed
SA537475	ATCC_7	BX62 Goat	BX62 Silage feed
SA537476	ATCC_10	BX62 Goat	BX62 Silage feed
SA537477	ATCC_9	BX62 Goat	BX62 Silage feed
SA537478	ATCC_8	BX62 Goat	BX62 Silage feed
SA537479	ATCC_1	BX62 Goat	BX62 Silage feed
SA537480	ATCC_6	BX62 Goat	BX62 Silage feed
SA537481	ATCC_5	BX62 Goat	BX62 Silage feed
SA537482	ATCC_4	BX62 Goat	BX62 Silage feed
SA537483	ATCC_3	BX62 Goat	BX62 Silage feed
SA537484	ATCC_11	BX62 Goat	BX62 Silage feed
SA537485	CK_10	CK Goat	CK Silage feed
SA537486	CK_2	CK Goat	CK Silage feed
SA537487	CK_3	CK Goat	CK Silage feed
SA537488	CK_4	CK Goat	CK Silage feed
SA537489	CK_5	CK Goat	CK Silage feed
SA537490	CK_6	CK Goat	CK Silage feed
SA537491	CK_7	CK Goat	CK Silage feed
SA537492	CK_8	CK Goat	CK Silage feed
SA537493	CK_9	CK Goat	CK Silage feed
SA537494	CK_1	CK Goat	CK Silage feed

Showing results 1 to 20 of 20

Showing results 1 to 20 of 20

Collection:

Collection ID:	CO004699
Collection Summary:	Healthy mid-lactation Guanzhong dairy goats (Capra hircus) were randomly assigned to 2 experimental groups and were offered diets containing 40% concentrate and 60% alfalfa silage inoculated with nothing (Control group, CK) or inoculated with L. plantarum BX62 (BX62 group). On Day 60, all experimental goats fasted for 12 h and then were transported to an abattoir for slaughter. The rumen was taken out and rumen content was immediately mixed and then the ruminal fluid sample was strained through four layers of sterile gauze and transferred to liquid nitrogen for flavonoid target determination.
Sample Type:	Digestive fluid
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR004715
Treatment Summary:	On Day 60, all experimental goats fasted for 12 h and then were transported to an abattoir for slaughter. The rumen content was immediately mixed and then the ruminal fluid sample was strained through four layers of sterile gauze and transferred to liquid nitrogen for flavonoid target determination.

Sample Preparation:

Sampleprep ID:	SP004712
Sampleprep Summary:	2 mL of rumen fluid sample was evaporated to dryness in a grinding tube, then mixed with pre-cooled (-20 °C) 70% (v/v) methanol aqueous solution at a 2:1 ratio, followed by homogenization at 60 Hz for 90 s. After 30 min of incubation on ice, the samples were centrifuged at 12,000 rpm for 3 min at 4 °C. A 300 µL aliquot of the supernatant was transferred to a new EP tube and dried using a vacuum centrifugal concentrator. The residue was reconstituted in 50 µL of 70% methanol aqueous solution, and following centrifugation, 30 µL of the supernatant was analyzed by HPLC (Vanquish Core HPLC, Thermo Scientific, USA) coupled with tandem mass spectrometry (Q Exactive HF-X MS Orbitrap, Thermo Scientific, USA).

Sampleprep ID:

SP004712

Sampleprep Summary:

2 mL of rumen fluid sample was evaporated to dryness in a grinding tube, then mixed with pre-cooled (-20 °C) 70% (v/v) methanol aqueous solution at a 2:1 ratio, followed by homogenization at 60 Hz for 90 s. After 30 min of incubation on ice, the samples were centrifuged at 12,000 rpm for 3 min at 4 °C. A 300 µL aliquot of the supernatant was transferred to a new EP tube and dried using a vacuum centrifugal concentrator. The residue was reconstituted in 50 µL of 70% methanol aqueous solution, and following centrifugation, 30 µL of the supernatant was analyzed by HPLC (Vanquish Core HPLC, Thermo Scientific, USA) coupled with tandem mass spectrometry (Q Exactive HF-X MS Orbitrap, Thermo Scientific, USA).

Combined analysis:

Analysis ID	AN007603
Chromatography ID	CH005766
MS ID	MS007300
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Thermo Vanquish
Column	Waters ACQUITY UPLC CSH C18 (50mm × 2.1mm; 1.7um)
MS Type	ESI
MS instrument type	Orbitrap
MS instrument name	Thermo Orbitrap Exploris 120
Ion Mode	UNSPECIFIED
Units	ng/mL

Chromatography:

Chromatography ID:	CH005766
Instrument Name:	Thermo Vanquish
Column Name:	Waters ACQUITY UPLC CSH C18 (50mm × 2.1mm; 1.7um)
Column Temperature:	40
Flow Gradient:	10–50% B (0–8 min), 50–80% B (8–9 min), 80–95% B (9–11 min), 95-95% B (11–12 min), 95-10% B (12–12.01 min), and re-equilibration at 10% B (12.01–14 min)
Flow Rate:	0.4 mL/min
Sample Injection:	1 μL
Solvent A:	100% water; 10mM ammonium acetate
Solvent B:	100% acetonitrile
Chromatography Type:	Reversed phase

MS:

MS ID:	MS007300
Analysis ID:	AN007603
Instrument Name:	Thermo Orbitrap Exploris 120
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Mass spectrometry ion source parameters were set as follows: spray voltage at 3,200 V, capillary temperature at 320°C, sheath gas flow rate at 30 arbitrary units (arb), auxiliary gas flow at 10 arb, and auxiliary gas heater temperature at 400°C.
Ion Mode:	UNSPECIFIED