| Metadata details for analysis AN000410 | |
|---|---|
| Study ID | ST000258 |
| Analysis ID | AN000410 |
| Study Title | Metabolic contribution of pSymA and pSymB megaplasmid/chromid for multipartite Sinorhizobium meliloti cultured in minimal M9 medium |
| Institute | McMaster University |
| Species | Sinorhizobium meliloti |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Bruker MicrOTOF II |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 1200 RR Series II |
| Chromatography Type | HILIC |
| Chromatography Column | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| Solvent A | 100% acetonitrile |
| Solvent B | 100% water;10 mM ammonium acetate, adjust to pH 3 with formic acid |
| Gradient | 0-0.5 min, 95%A; 0.5-12.5 min, 35%A; 12.5-13.0 min, 35%A; 13.0-14.0 min, 95%A; 14.0-24.0 min, 95%A |
| Flow rate | 200 ?L/min |
| Column Temperature | 40C |
| Retention time units | Minutes |
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