| Metadata details for analysis AN001003 | |
|---|---|
| Study ID | ST000657 |
| Analysis ID | AN001003 |
| Study Title | mTOR regulates metabolic adaptation of APCs in the lung microenvironment and controls the outcome of allergic inflammation |
| Institute | Emory University |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | HILIC |
| Chromatography Column | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| Solvent A | 100% water(A), 100% acetonitrile(B), 100% water; 2% formic acid(C) |
| Solvent B | 100% water(A), 100% acetonitrile(B), 100% water; 2% formic acid(C) |
| Gradient | 0-2min, 10%A-80%B-10%C; 2-7min, 80%A-10%B-10%C; 7-10min, 80%A-10%B-10%C. |
| Flow rate | .350 ul/min |
| Column Temperature | 30 |
| Retention time units | Seconds |