| Metadata details for analysis AN001460 | |
|---|---|
| Study ID | ST000898 |
| Analysis ID | AN001460 |
| Study Title | TAp73 is a marker of glutamine addiction in medulloblastoma |
| Institute | Queen Mary University of London |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Waters Synapt G2 |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Waters Acquity I-Class |
| Chromatography Type | HILIC |
| Chromatography Column | Waters Acquity BEH Amide (50 x 2.1mm,1.7um) |
| Solvent A | 90% acetonitrile/10% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B | 50% acetonitrile/50% water; 0.1% formic acid; 10 mM ammonium formate |
| Gradient | Non-linear gradient elution profile from 100 % A to 100 % B was used. In detail; 100% A was kept for 0.5 min then decreased non-linearly (slope-factor 8 in MassLynx) over 12.5 min to 100% B, 100% B was held for 3 min followed by 7 min at 100 % A to re-equilibrate the column for a total run-time of 23 min. |
| Flow rate | 0.3 ml/min |
| Column Temperature | 40°C |
| Retention time units | Seconds |
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