| Metadata details for analysis AN001967 | |
|---|---|
| Study ID | ST001185 |
| Analysis ID | AN001967 |
| Study Title | Genetic and metabolic characterization of bioengineered human fatty liver tissue with modified SIRT1 expression |
| Institute | University of Pittsburgh |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Thermo Fisher Accucore C18 (100 x 2.1mm,5um) |
| Solvent A | 50% water/50% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate |
| Solvent B | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate |
| Gradient | The gradient started at 0%B and increased to 50%B from 2-10 min following a second increase to 95%B from 10-47 min. The gradient was held for 4 min at 95%B before increasing to 100%B at 51 min for a 6 min wash. At 57 min the system was returned to initial conditions to equilibrate before the next injection. Total run time was 60 min. |
| Flow rate | 0.2 mL/min |
| Column Temperature | |
| Retention time units | Minutes |
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