| Metadata details for analysis AN002084 | |
|---|---|
| Study ID | ST001255 |
| Analysis ID | AN002084 |
| Study Title | Immunomodulatory activity of hyaluronidase is associated with metabolic adaptations during acute inflammation |
| Institute | Sao Paulo University |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | ABI Sciex 5600 TripleTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Shimadzu Nexera X2 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Ascentis Express C18 (100 x 4.6mm,2.7um) |
| Solvent A | 70% water/30% acetonitrile; 0.02% acetic acid, pH 5.8(adjusted with ammonium hydroxide) |
| Solvent B | 70% acetonitrile/30% isopropanol |
| Gradient | 0 to 2.0 min, 0% B; 2.0 to 5.0 min, 15% B; 5.0 to 8.0 min, 20% B; 8.0 to 11.0 min, 35% B; 11.0 to 15.0 min, 70% B; and 15.0 to 19 min, 100% B. At 19.0 min, the gradient returned to the initial condition of 0% B, and the column was re-equilibrated until 25.0 min. |
| Flow rate | 0.6ml/min |
| Column Temperature | |
| Retention time units | Seconds |
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