| Metadata details for analysis AN002100 | |
|---|---|
| Study ID | ST001264 |
| Analysis ID | AN002100 |
| Study Title | Antibiotics and Dietary Minerals Lipidomics |
| Institute | University of California, Davis |
| Species | Rattus norvegicus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish Focused UHPLC |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Solvent A | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Gradient | 0 min 15% (B); 0−2 min 30% (B); 2−2.5 min 48% (B); 2.5−11 min 82% (B); 11−11.5 min 99% (B); 11.5−12 min 99% (B); 12−12.1 min 15% (B); and 12.1−15 min 15% (B) |
| Flow rate | 0.6 mL/min |
| Column Temperature | 65 |
| Retention time units | Minutes |
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