| Metadata details for analysis AN002211 | |
|---|---|
| Study ID | ST001328 |
| Analysis ID | AN002211 |
| Study Title | Multiplatform, non-targeted analysis of lung extracts of uninfected and Mtb-infected C57BL/6 mice at 4 and 9 weeks p.i. |
| Institute | Universidad CEU San Pablo |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6520 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 1200 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent Poroshell 120 EC-C8 (150 x 2.1mm,2.7um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 85% methanol/15% isopropanol; 0.1 % formic acid |
| Gradient | Initial conditions at time 0 were 82 % B, increasing to 96 % B in 30 min. This was then held until 38 min. The gradient then increased to 100 % B by 38.5 min and held until 40.5 min. The conditions were then returned to the starting conditions by 42 min, followed by an 8 min re-equilibration time. The total run time of the method was 50 min. |
| Flow rate | 0.4mL·min-1 |
| Column Temperature | 60 |
| Retention time units | Minutes |
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