| Metadata details for analysis AN003108 | |
|---|---|
| Study ID | ST001911 |
| Analysis ID | AN003108 |
| Study Title | A high-fat diet induced obesity murine model treated with bitter melon (Momordica charantia) |
| Institute | University of Colorado Anschutz Medical Campus |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6210 TOF |
| MS Instrument Type | TOF |
| Chromatography Instrument Name | Agilent 1290 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 60% isopropanol/36% acetonitrile/4% water; 0.1% formic acid |
| Gradient | 0-1 minute 30-70% B, 1-7.92 minutes 70-100% B, 7.92-10.4 minutes 100% B, 10.4-10.5 minutes 100-30% B, followed by column re-equilibration with 30% B from 10.5-15.1 minutes. |
| Flow rate | 0.7 mL/min |
| Column Temperature | 60 |
| Retention time units | Minutes |
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