| Metadata details for analysis AN003238 | |
|---|---|
| Study ID | ST001987 |
| Analysis ID | AN003238 |
| Study Title | Transcriptomic and lipidomic analysis unravels the response of Faecalibacterium prausnitzii to calcium palmitate |
| Institute | University of Groningen |
| Species | Faecalibacterium prausnitzii |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Bruker maXis Impact qTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Waters Acquity |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| Solvent A | 40% water/60% acetonitrile; 0.005% formic acid; 10 mM ammonium formate |
| Solvent B | 90% isopropanol/10% acetonitrile; 0.01% formic acid; 10 mM ammonium formate |
| Gradient | 0- 2 min from 40 to 43% eluent B, 2-12 min from 50 to 54% eluent B, 12-18 min from 70 to 99% eluent B, and 18-20 min 40% eluent B. |
| Flow rate | 0.4 mL/min |
| Column Temperature | 55 |
| Retention time units | Seconds |
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