| Metadata details for analysis AN003573 | |
|---|---|
| Study ID | ST002181 |
| Analysis ID | AN003573 |
| Study Title | Piperaquine-resistant PfCRT mutations differentially impact drug transport, hemoglobin catabolism and parasite physiology in Plasmodium falciparum asexual blood stages. |
| Institute | Pennsylvania State University |
| Species | Plasmodium falciparum |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | ABI Sciex 5600 TripleTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Prominence 20 UFLCXR |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | The initial conditions were 97% A and 3 % B, increasing to 45% B at 10 min, 75% B at 12 min where it was held at 75% B until 17.5 min before returning to the initial conditions. |
| Flow rate | 250 ul/min |
| Column Temperature | 55 |
| Retention time units | Minutes |