| Metadata details for analysis AN003597 | |
|---|---|
| Study ID | ST002198 |
| Analysis ID | AN003597 |
| Study Title | Untargeted metabolomics of Pinus pinaster needles under heat and drought stress |
| Institute | Universidad de Oviedo |
| Species | Pinus pinaster |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Bruker Impact II HD |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Phenomenex Luna Omega Polar C18 (100 x 2.1 mm,1.7um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | Gradient elution chromatography was performed starting with 100 % A to 98 % A in 1 min; hold for 9 min, gradient to 60 % A in 21 min, gradient to 45 % A in 5 min; hold for 2 min, gradient to 5 % A in 3 min; hold 5 min, return to initial conditions in 3 min and equilibrate for 5 min (total run time: 53 min) |
| Flow rate | 0.1 mL/min |
| Column Temperature | 30 ºC |
| Retention time units | Minutes |
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