| Metadata details for analysis AN003688 | |
|---|---|
| Study ID | ST002258 |
| Analysis ID | AN003688 |
| Study Title | Lipidomic profiling reveals age-dependent changes in complex plasma membrane lipids that regulate neural stem cell aging (Part 2) |
| Institute | Stanford University |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 RS |
| Chromatography Type | Reversed phase |
| Chromatography Column | Thermo Accucore C18 (150 x 2.1mm,2.6m) |
| Solvent A | 60% acetonitrile/40% water; 0.1% formic acid; 10mM ammonium acetate |
| Solvent B | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10mM ammonium acetate |
| Gradient | 30% B for 3min, 30-43% in 2min, 43-55% B in 0.1min, 55-65% in 10min, 65-85% B in 6min, 85-100% B in 2min and 100% B for 5min. |
| Flow rate | 0.4ml/min |
| Column Temperature | 45 |
| Retention time units | Minutes |
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