| Metadata details for analysis AN003736 | |
|---|---|
| Study ID | ST002285 |
| Analysis ID | AN003736 |
| Study Title | Multiplatform mass spectrometry-based analysis of Leishmania donovani infected macrophages at different time points after infection |
| Institute | Universidad CEU San Pablo |
| Species | Homo sapiens + Leishmania donovani |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6545 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 1200 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent Zorbax Extend C18 RRHT (50 x 2.1mm,1.8 um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | Initial conditions at time 0 were 5% B, held until 1 min. Next, the percentage of organic phase was gradually increased up to 80% B at 7 min and subsequently until 100% B at 11.5 min. The conditions were then returned to the starting conditions by 12 min, followed by a 3 min re-equilibration time. The total run time of the method was 15 min. |
| Flow rate | 0.6 mL/min |
| Column Temperature | 60 ºC |
| Retention time units | Minutes |
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