Metadata details for analysis AN003769
Study IDST002307
Analysis IDAN003769
Study TitleMetabolomics profiling of the secondary fractions 4 of bacterial culture supernatants.
InstituteUniversity of Connecticut
SpeciesBacillus megaterium, Enterococcus faecium
Ion_modePOSITIVE
MS typeESI
MS Instrument NameWaters Synapt-G2-Si
MS Instrument TypeQTOF
Chromatography Instrument NameACQUITY UPLC H-Class
Chromatography TypeReversed phase
Chromatography ColumnWaters CORTECS UPLC T3 (150 x 2.1mm,1.6um)
Solvent A100% water; 0.1% formic acid
Solvent B100% acetonitrile; 0.1% formic acid
GradientThe gradient (% for Solvent B at runtime) method was 1% from 0 to 1 minute, 20% at 15 minutes, 90% from 15.1 to 19 minutes, and 1% from 19.1 to 21 minutes.
Flow rate300 µL/min
Column Temperature40
Retention time unitsMinutes
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