| Metadata details for analysis AN003820 | |
|---|---|
| Study ID | ST002338 |
| Analysis ID | AN003820 |
| Study Title | Interplay Between Cruciferous Vegetables and the Gut Microbiome: A Multi-Omic Approach |
| Institute | Oregon State University |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | ABI Sciex 5600 TripleTOF |
| MS Instrument Type | Triple TOF |
| Chromatography Instrument Name | Shimadzu Nexera |
| Chromatography Type | Reversed phase |
| Chromatography Column | GL Sciences Inertsil1 Phenyl-3 (4.6 x 150mm,5um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% methanol; 0.1% formic acid |
| Gradient | The gradient started with 5% B and was held for 1 min at 5% B, followed by a 11-min linear gradient from 5% to 30% B. The gradient was increased linearly to 100% B at 23 min, held for 5 min at 100% B and, finally, stepped back to 5% B to equilibrate the column. |
| Flow rate | 0.4ml/min |
| Column Temperature | 50 |
| Retention time units | Minutes |