| Metadata details for analysis AN003826 | |
|---|---|
| Study ID | ST002342 |
| Analysis ID | AN003826 |
| Study Title | The gut microbiome has sexually dimorphic effects on bone tissue energy metabolism and multiscale bone quality in C57BL/6J mice |
| Institute | Montana State University |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6538 QTOF |
| MS Instrument Type | Single quadrupole |
| Chromatography Instrument Name | Agilent 1290 |
| Chromatography Type | HILIC |
| Chromatography Column | Cogent Diamond Hydride (150 × 2.1mm,4um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | 100 to 25% solvent B over 17 minutes, 0 to 70% solvent A over 20 minutes, at 21 minutes, B increases from 25% to 100% till 25 minutes while A decreases from 70% to 0% till 25 minutes. The last 4 minutes act as a wash. |
| Flow rate | 0.400 mL/min |
| Column Temperature | |
| Retention time units | Minutes |