| Metadata details for analysis AN003915 | |
|---|---|
| Study ID | ST002402 |
| Analysis ID | AN003915 |
| Study Title | Four-dimensional trapped ion mobility spectrometry lipidomics for high throughput clinical profiling of human blood samples |
| Institute | University Medical Center of Mainz |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Bruker timsTOF |
| MS Instrument Type | timsTOF |
| Chromatography Instrument Name | Bruker Elute UHPLC |
| Chromatography Type | Reversed phase |
| Chromatography Column | Phenomenex Luna Omega C18 (100 x 2.1mm,1.6um) |
| Solvent A | 50% methanol/50% water; 0.1% formic acid; 7.5 mM ammonium formate |
| Solvent B | 20% methanol/80% isopropanol; 0.1% formic acid; 7.5 mM ammonium formate |
| Gradient | The LC method consisted of a 20 min runtime with the following gradient: T0 to T1 – mobile phase A at 60% and mobile phase B at 40% which was then elevated to 90% mobile phase B and 10% mobile phase by T16 and up to 99% mobile phase B by T16.5 and then escalated back to 40% mobile phase B by T20 |
| Flow rate | 200 µl/min |
| Column Temperature | 45 |
| Retention time units | Minutes |
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