| Metadata details for analysis AN003961 | |
|---|---|
| Study ID | ST002432 |
| Analysis ID | AN003961 |
| Study Title | Metabolic impacts of metformin to seasonal influenza vaccination: a pilot study of drug interaction with the immune response |
| Institute | The Jackson Laboratory for Genomic Medicine |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Orbitrap ID-X Tribrid |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Scientific Transcend Duo LX-2 UHPLC |
| Chromatography Type | Reversed phase |
| Chromatography Column | Thermo Hypersil GOLD RP (50 x 2.1mm, 3um) |
| Solvent A | 10 mM ammonium acetate in acetonitrile:water (95:5, v/v) with 0.1% acetic acid |
| Solvent B | 10 mM ammonium acetate in acetonitrile:water (50:50, v/v) with 0.1% acetic acid |
| Gradient | 0-0.1 min: 0% B, 0.10-1.9 min: 60% B, 1.9-5.0 min: 98% B, and 5 min cleaning and column equilibration |
| Flow rate | 0.4 ml/min |
| Column Temperature | 45 |
| Retention time units | Seconds |
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