| Metadata details for analysis AN004031 | |
|---|---|
| Study ID | ST002470 |
| Analysis ID | AN004031 |
| Study Title | Linking bacterial metabolites to disease-associated microbes to uncover mechanisms of host-microbial interactions in intestinal inflammation. Human plasma profiling |
| Institute | Broad Institute of MIT and Harvard |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Shimadzu Nexera X2 |
| Chromatography Type | HILIC |
| Chromatography Column | Phenomenex Luna NH2 (150 x 2.1mm, 3um) |
| Solvent A | 100% water; 20 mM ammonium acetate; 20 mM ammonium hydroxide |
| Solvent B | 75% acetonitrile/25% methanol; 10 mM ammonium hydroxide |
| Gradient | The column was eluted with initial conditions of 10% mobile phase A and 90% mobile phase B followed by a 10 min linear gradient to 100% mobile phase A. |
| Flow rate | 400 µL/min |
| Column Temperature | 30C |
| Retention time units | Minutes |
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