| Metadata details for analysis AN004036 | |
|---|---|
| Study ID | ST002471 |
| Analysis ID | AN004036 |
| Study Title | Linking bacterial metabolites to disease-associated microbes to uncover mechanisms of host-microbial interactions in intestinal inflammation. Human stool profiling |
| Institute | Broad Institute of MIT and Harvard |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Shimadzu Nexera X2 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC BEH C18 (150 x 1.7mm,2.1um) |
| Solvent A | 100% water; 0.01% formic acid |
| Solvent B | 100% acetonitrile; 0.01% acetic acid |
| Gradient | The column was eluted isocratically at a flow rate of 450 µL/min with 20% mobile phase A for 3 minutes followed by a linear gradient to 100% mobile phase B over 12 minutes. |
| Flow rate | 450 µL/min |
| Column Temperature | 45C |
| Retention time units | Minutes |
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