| Metadata details for analysis AN004053 | |
|---|---|
| Study ID | ST002483 |
| Analysis ID | AN004053 |
| Study Title | Brain metabonomics of rats in CO and ASO groups from 4500m were compared. |
| Institute | Bao Feng Key Laboratory of Genetics and Metabolism |
| Species | Rattus norvegicus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Waters Xevo-G2-XS |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Waters Acquity I-Class |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters Acquity BEH C8 (100 x 2.1mm, 1.7um) |
| Solvent A | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Gradient | The injected 1 µL sample was initially eluted with 40% B, graded linearly to 43% B in 2 minutes, and then increased to 50% B in 0.1 minutes. In the next 9.9 minutes, the gradient was further increased to 54% B and then increased to 70% in 0.1 minutes. In the last part of the gradient, the amount of B was increased to 99% in 5.9 minutes. Finally, the column was balanced for 1.9 minutes before the next injection, and solution B returned to 40% in 0.1 minutes. |
| Flow rate | 0.4 mL/min |
| Column Temperature | 550 ℃ |
| Retention time units | Minutes |
| Study ID | ST002483 |
| Analysis ID | AN004053 |
| Study Title | Brain metabonomics of rats in CO and ASO groups from 4500m were compared. |
| Institute | Bao Feng Key Laboratory of Genetics and Metabolism |
| Species | Rattus norvegicus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Waters Xevo-G2-XS |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Waters Acquity I-Class |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters Acquity BEH C8 (100 x 2.1mm, 1.7um) |
| Solvent A | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Gradient | The injected 1 µL sample was initially eluted with 40% B, graded linearly to 43% B in 2 minutes, and then increased to 50% B in 0.1 minutes. In the next 9.9 minutes, the gradient was further increased to 54% B and then increased to 70% in 0.1 minutes. In the last part of the gradient, the amount of B was increased to 99% in 5.9 minutes. Finally, the column was balanced for 1.9 minutes before the next injection, and solution B returned to 40% in 0.1 minutes. |
| Flow rate | 0.4 mL/min |
| Column Temperature | 550 ℃ |
| Retention time units | Minutes |
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