| Metadata details for analysis AN004093 | |
|---|---|
| Study ID | ST002495 |
| Analysis ID | AN004093 |
| Study Title | Disrupted intestinal microbiota contributes to the pathogenesis of anorexia nervosa (Part 2) |
| Institute | Örebro University |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 7200 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 7890B |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Solvent A | 70% water/30% methanol |
| Solvent B | 100% methanol |
| Gradient | The flow rate was set at 0.4 mLmin-1 with the elution gradient as follows: 0-1.5 min, mobile phase B was increased from 5% to 30%; 1.5-4.5 min, mobile phase B increased to 70%; 4.5-7.5 min, mobile phase B increased to 100% and held for 5.5 min. A post-time of 5 min was used to regain the initial conditions for the next analysis. The total run time per sample was 18 min |
| Flow rate | 0.4ml/min |
| Column Temperature | 70 |
| Retention time units | Minutes |
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