| Metadata details for analysis AN004121 | |
|---|---|
| Study ID | ST002504 |
| Analysis ID | AN004121 |
| Study Title | Lipid droplets and peroxisomes are co-regulated to drive lifespan extension in response to mono-unsaturated fatty acids |
| Institute | Stanford University |
| Species | Caenorhabditis elegans |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 RS |
| Chromatography Type | Reversed phase |
| Chromatography Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
| Solvent A | 60% acetonitrile/40% water; 1mM ammonium formate; 0.1% formic acid |
| Solvent B | 90% isopropanol/10% acetonitrile; 1mM ammonium formate; 0.1% formic acid |
| Gradient | The gradient profile used was 30% B for 3 minutes, 30–43% B over 5 minutes, 43–50% B over 1 minute, 55–90% B over 9 minutes, 90-99% B over 9 minutes and 99% B for 5 minutes. |
| Flow rate | 0.2 ml/min |
| Column Temperature | 30 |
| Retention time units | Minutes |
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