| Metadata details for analysis AN004166 | |
|---|---|
| Study ID | ST002532 |
| Analysis ID | AN004166 |
| Study Title | Nontargeted metabolomics analysis on kidney tissue treated with cisplatin |
| Institute | Children's Hospital of Nanjing Medical University |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Focus |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Ultimate 3000 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Solvent A | 0.1% formic acid in water and 0.1% formic acid in acetonitrile |
| Solvent B | 5 mM ammonium formate in water and acetonitrile |
| Gradient | 0~1 min, 2% B/D; 1~9 min, 2%~50% B/D; 9~12 min, 50%~98% B/D; 12~13.5 min, 98% B/D; 13.5~14 min, 98%~2% B/D; 14~20 min, 2% D-positive model (14~17 min, 2% B-negative model) |
| Flow rate | 0.25 mL/min |
| Column Temperature | 40 |
| Retention time units | Minutes |
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