| Metadata details for analysis AN004235 | |
|---|---|
| Study ID | ST002570 |
| Analysis ID | AN004235 |
| Study Title | Evaluation of Extraction Parameters for the Analysis of Lipid Classes in Plants |
| Institute | University of Campinas |
| Species | Pitcairnia flammea |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Scientific UltiMate™ 3000 UHPLC RSLCnano system |
| Chromatography Type | Reversed phase |
| Chromatography Column | Supelco Sigma-Aldrich Titan C18 column (100 mm x 2.1 mm x 1.9 µm) |
| Solvent A | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B | 10% acetonitrile/90% isopropanol; 10 mM ammonium acetate |
| Gradient | 250 µL/min: 0-2 min: 40% B, 2-3 min: 50% B, 3-6 min: 50% B, 6.1-8 min: 70% B, 8-9 min: 100% B, 9-11 min: 100% B, 11-12 min: 40% B, 12-14 min: 40% B. |
| Flow rate | 250 µL/min |
| Column Temperature | 40 |
| Retention time units | Minutes |
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