| Metadata details for analysis AN004402 | |
|---|---|
| Study ID | ST002715 |
| Analysis ID | AN004402 |
| Study Title | Metabolic alteration of MCF-7 cells upon indirect exposure to E. coli secretome: A model of studying the microbiota effect on human breast tissue |
| Institute | King Saud University |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Waters Xevo G2-S |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Waters Acquity UPLC |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters XSelect HSS C18 (100 × 2.1mm, 2.5um) |
| Solvent A | 0.1% formic acid in dH2O |
| Solvent B | 0.1% formic acid in 50% ACN:MeOH |
| Gradient | 0–16 min 95%–5% A, 16–19 min 5% A, 19–20 min 5%–95% A, and 20–22 min, 95%– 95% A |
| Flow rate | 300 μl/min. |
| Column Temperature | 55 |
| Retention time units | Minutes |
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