| Metadata details for analysis AN004440 | |
|---|---|
| Study ID | ST002738 |
| Analysis ID | AN004440 |
| Study Title | Metabolic energy crisis triggers NLRC5/mTOR-mediated ROS modulation and targeting to the cell membrane for lysis |
| Institute | St. jude Children's research hospital |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | Waters XBridge BEH Amide (150 x 2.1mm, 2.5um) |
| Solvent A | water supplemented with 10 mM ammonium acetate |
| Solvent B | acetonitrile |
| Gradient | 0–16.5 min, 1 to 50% B; 16.5–18 min, 50 to 99% B; 18–36 min, 99% B; 36–39 min, 99 to 1% B; 39–45 min, 1% B. |
| Flow rate | 100 μL/min |
| Column Temperature | 40 |
| Retention time units | Minutes |
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