| Metadata details for analysis AN004458 | |
|---|---|
| Study ID | ST002748 |
| Analysis ID | AN004458 |
| Study Title | HER2 overexpression initiates breast tumorigenesis non-cell autonomously by inducing oxidative stress in the tissue microenvironment |
| Institute | The University of Manchester |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6600 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Thermo-Fisher Ultimate 3000 HPLC |
| Chromatography Type | HILIC |
| Chromatography Column | Agilent Poroshell 120 HILIC-Z |
| Solvent A | 100% water; 10 mM ammonium acetate adjusted to pH 9 with ammonium hydroxide and 20 µM medronic acid |
| Solvent B | 85% acetonitrile/15% water; 10 mM ammonium acetate adjusted to pH 9 with ammonium hydroxide and 20 µM medronic acid |
| Gradient | flow rate of 0.25 ml/min, starting at 96 % B for 2 minutes, ramping to 65 % B over 20 min, hold at 65 % B for 2 min, then back to 96 % B |
| Flow rate | 0.25 ml/min |
| Column Temperature | 40 |
| Retention time units | Seconds |
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