| Metadata details for analysis AN004467 | |
|---|---|
| Study ID | ST002753 |
| Analysis ID | AN004467 |
| Study Title | Comparative analyses of metabolome for the intact mammary ducts of 8-week-old MMTV-Neu-IRES-Cre mice versus their wildtype littermates |
| Institute | Manchester Institute of Biotechnology, University of Manchester |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | ABI Sciex 6600 TripleTOF |
| MS Instrument Type | Triple TOF |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | HILIC |
| Chromatography Column | Agilent Poroshell 120 HILIC-Z column (150 mm length, 2.1 mm diameter and 2.7 μm particle size) |
| Solvent A | 100% water; 10 mM ammonium acetate (pH 9); 20 µM medronic acid |
| Solvent B | 85% acetonitrile/ 15% water; 10 mM ammonium acetate (pH 9); 20 µM medronic acid |
| Gradient | starting at 96 % B for 2 minutes, ramping to 65 % B over 20 min, hold at 65 % B for 2 min, then back to 96 % B |
| Flow rate | 0.25 ml/min |
| Column Temperature | 50 |
| Retention time units | Minutes |
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