| Metadata details for analysis AN004538 | |
|---|---|
| Study ID | ST002789 |
| Analysis ID | AN004538 |
| Study Title | Metabolomic analysis of particulate matter in the NPSG during a 2017 cruise on the R/V Kilo Moana as part of the MESOSCOPE program |
| Institute | University of Washington |
| Species | Natural mixed marine microbial community |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Waters Acquity I-Class |
| Chromatography Type | HILIC |
| Chromatography Column | SeQuant ZIC-HILIC (150 x 2.1mm, 5um) |
| Solvent A | 85% acetonitrile/15% water |
| Solvent B | 15% acetonitrile/85% water |
| Gradient | 100% A for 2 minutes, ramped to 64% B over 18 minutes, ramped to 100% B over 1 minute, held at 100% B for 5 minutes, and equilibrated at 100% A for 25 minutes (50 minutes total) |
| Flow rate | 0.15 mL/min |
| Column Temperature | 30 |
| Retention time units | Minutes |
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