| Metadata details for analysis AN004540 | |
|---|---|
| Study ID | ST002790 |
| Analysis ID | AN004540 |
| Study Title | Community metabolomes reflect taxon-specific fingerprints of phytoplankton and heterotrophic bacteria in the ocean (expanded) |
| Institute | University of Washington |
| Species | Marine plankton |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Waters Acquity I-Class |
| Chromatography Type | HILIC |
| Chromatography Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
| Solvent A | 85% acetonitrile/15% water; 10 mM ammonium carbonate |
| Solvent B | 15% acetonitrile/85% water; 10 mM ammonium carbonate |
| Gradient | 100% A for 2 minutes, ramped to 64% B over 18 minutes, ramped to 100% B over 1 minute, held at 100% B for 5 minutes, and equilibrated at 100% A for 25 minutes (50 minutes total) |
| Flow rate | 0.15mL/min |
| Column Temperature | 30 |
| Retention time units | Minutes |