| Metadata details for analysis AN004663 | |
|---|---|
| Study ID | ST002845 |
| Analysis ID | AN004663 |
| Study Title | Methylprednisolone therapy induces differential metabolic trajectories in severe COVID-19 patients |
| Institute | Federal University of Goiás |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Agilent 1220 Infinity |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent Zorbax Eclipse Plus C18 (150 x 4.6mm,3.5um) |
| Solvent A | 100% water; 0.5% formic acid; 5 mM of ammonium formate |
| Solvent B | 100% acetonitrile |
| Gradient | gradient elution started with 20% (B) for 5 min, then linearly increased to 100% (B) in 30 min and kept constant for 8 min in 100% (B). The eluent was restored to the initial conditions in 4 minutes to re-equilibrate the column and held for the remaining 8 minutes. |
| Flow rate | 0.5 mL/min |
| Column Temperature | 35 |
| Retention time units | Minutes |
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