| Metadata details for analysis AN004667 | |
|---|---|
| Study ID | ST002849 |
| Analysis ID | AN004667 |
| Study Title | Lipidomics for unlinked CD1 proteins |
| Institute | Brigham and Women's Hospital |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6520 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 1200 |
| Chromatography Type | Normal phase |
| Chromatography Column | First through a MetaGuard guard column (2 mm, 3 μm, Varian, A0542-MG2) and then a MonoChrom Diol column (150 X 2 mm, 3μm, Varian, A0542150X020) |
| Solvent A | 70% isopropanol, 30% methanol, 0.1% formic acid, and 0.05% ammonium hydroxide |
| Solvent B | 70% hexanes, 30% isopropanol, 0.1% formic acid, and 0.05% ammonium hydroxide |
| Gradient | The binary gradient was monitored with solvent B with 100% at 0-10 min, 50% at 17-22 min, 0% at 30-35 min, and 100% at 40-50 min, followed by an additional 0% for 6 min post-run for regeneration. |
| Flow rate | 0.7 ml/min |
| Column Temperature | 22 |
| Retention time units | Minutes |
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