| Metadata details for analysis AN004668 | |
|---|---|
| Study ID | ST002850 |
| Analysis ID | AN004668 |
| Study Title | Bap1 Promotes Osteoclast Function by Metabolic Reprogramming |
| Institute | Washington University in St. Louis |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Orbitrap ID-X Tribrid |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish Flex UHPLC Systems |
| Chromatography Type | HILIC |
| Chromatography Column | HILICON iHILIC-(P) Classic (100 x 2.1mm,5um) |
| Solvent A | 20 mM ammonium bicarbonate, 0.1% ammonium hydroxideand 2.5 μM medronic acid in water:acetonitrile (95:5) |
| Solvent B | acetonitrile:water (95:5) |
| Gradient | 0–1 min: 90% B, 1–12 min: 90-35% B, 12–12.5 min: 35-25% B, 12.5–14.5 min: 25% B |
| Flow rate | 250 uL/min |
| Column Temperature | 45 |
| Retention time units | Seconds |
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