| Metadata details for analysis AN004805 | |
|---|---|
| Study ID | ST002929 |
| Analysis ID | AN004805 |
| Study Title | Hypoxia-inducible factor-1a (HIF1a) physiology in mouse skeletal muscle |
| Institute | Cleveland Clinic |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Focus |
| MS Instrument Type | QTRAP |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | Time (min.) Flow Rate (mL/min) %B 0.2 0.3 5 1.0 0.3 15 8.0 0.3 95 10.0 0.3 95 12.0 0.3 5 15.0 0.3 5 |
| Flow rate | 0.3 mL/min |
| Column Temperature | 60 |
| Retention time units | Seconds |
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