| Metadata details for analysis AN004808 | |
|---|---|
| Study ID | ST002930 |
| Analysis ID | AN004808 |
| Study Title | Role of Hypoxia-inducible factor-1a (HIF1a)in Skeletal Muscle Physiology (C2C12 myotube model) |
| Institute | Cleveland Clinic |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Focus |
| MS Instrument Type | QTRAP |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Solvent A | 100% water;10 mM ammonium acetate; 0.125% acetic acid |
| Solvent B | 5% water/95% acetonitrile;10 mM ammonium acetate; 0.125% acetic acid |
| Gradient | Time (min.) Flow Rate (mL/min) %B 0.0 0.2 100 2.0 0.2 70 4.0 0.2 60 5.5 0.2 60 10.5 0.2 50 11.5 0.2 50 12.0 0.2 100 16.0 0.2 100 |
| Flow rate | 0.2 mL/min |
| Column Temperature | 40 |
| Retention time units | Seconds |
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