| Metadata details for analysis AN004833 | |
|---|---|
| Study ID | ST002947 |
| Analysis ID | AN004833 |
| Study Title | Hepatic lipid profiles of wild type and Cgref1-depleted mice of C57BL/6 strain obtained by an untargeted LC-MS/MS analysis |
| Institute | The University of Hong Kong |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Orbitrap Exploris 120 |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
| Solvent A | 10mM ammonium formate with 0.1% formic acid in acetonitrile and water, v/v 6:4 |
| Solvent B | 10mM ammonium formate with 0.1% formic acid in acetonitrile and IPA 1:9 |
| Gradient | The gradient started at 30% B and was increased to 43% B in 2 min, then increased to 55% B in 2.1 min, 65 % B in 12 min, 85% B in 18 min and 100 % B in 20 min, then held for 5min, and decreased linearly to 30% B for re-equilibration time at starting conditions. |
| Flow rate | 0.26 mL min−1 |
| Column Temperature | 45°C |
| Retention time units | Minutes |
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