| Metadata details for analysis AN004868 | |
|---|---|
| Study ID | ST002964 |
| Analysis ID | AN004868 |
| Study Title | Comprehensive Untargeted LC-MS Metabolomics Analysis of CYP19A1/PRLR/PTN Knockout iPSCs |
| Institute | The Jackson Laboratory for Genomic Medicine |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF-X Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Hypersil GOLD (50 x 2.1mm, 3um) |
| Solvent A | 60% ACN/water (10 mM ammonium formate, 0.1% formic acid) |
| Solvent B | 90% 2-propanol/ACN (10 mM ammonium formate, 0.1% formic acid) |
| Gradient | From 0 to 0.01 minutes, B is 15% at a flow rate of 400 µL/min; by 2.01 minutes, it's 30%, reaching 48% at 2.51 minutes, then 82% by 11 minutes, and it sharply rises to 99% at 11.50 minutes holding until 16.50 minutes when it peaks at 100%; from 17.50 to 20 minutes, it drops back to 15%, with the flow rate consistently maintained at 400 µL/min throughout. |
| Flow rate | 400µL/min |
| Column Temperature | 45 |
| Retention time units | Seconds |
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