| Metadata details for analysis AN004882 | |
|---|---|
| Study ID | ST002973 |
| Analysis ID | AN004882 |
| Study Title | Examine the through-filter recovery of metabolites extracted from a complex bacterial medium |
| Institute | Duke University |
| Species | |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Orbitrap Exploris 240 |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Solvent A | 100% water + 0.1% formic acid |
| Solvent B | 100% methanol + 0.1% formic acid |
| Gradient | From 0.5% B to 70% B in 4 minutes, from 70% B to 98% B in 0.5 minutes, and holding at 98% B for 0.9 minute before returning to 0.5% B in 0.2 minutes. |
| Flow rate | 0.350 mL/minute |
| Column Temperature | 40C |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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