| Metadata details for analysis AN004884 | |
|---|---|
| Study ID | ST002974 |
| Analysis ID | AN004884 |
| Study Title | Leishmania mexicana Promotes Pain-reducing Metabolomic Reprogramming In Cutaneous Lesions |
| Institute | The Ohio State University |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Scientific Ultimate WPS 3000 UHPLC system |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent Infinity Poroshell 120 SB-C18 (100 x 2 mm,2.7um) |
| Solvent A | H2O with 0.1% Formic acid |
| Solvent B | 100% acetonitrile |
| Gradient | an initial 2% solvent B with a linear ramp to 95% B at 15 min, holding at 95% B for 1 minutes, and back to 5% B from 17 min and equilibration of 5% B until min 30 |
| Flow rate | 200 µL/min flow rate |
| Column Temperature | 320 °C capillary temperature and 100 °C probe temperature |
| Retention time units | Minutes |
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