| Metadata details for analysis AN004898 | |
|---|---|
| Study ID | ST002980 |
| Analysis ID | AN004898 |
| Study Title | Water soluble metabolomics in mice upon loss of SHMT |
| Institute | Princeton University |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) |
| Solvent A | 95% water/5% acetonitrile with 20 mM ammonium acetate, 20 mM ammonium hydroxide, pH 9.4 |
| Solvent B | 100% acetonitrile |
| Gradient | 0 minutes, 85% B; 2 minutes, 85% B; 3 minutes, 80% B; 5 minutes, 80% B; 6 minutes, 75% B; 7 minutes, 75% B; 8 minutes, 70% B; 9 minutes, 70% B; 10 minutes, 50% B; 12 minutes, 50% B; 13 minutes, 25% B; 16 minutes, 25% B; 18 minutes, 0% B; 23 minutes, 0% B; 24 minutes, 85% B; 30 minutes, 85% B |
| Flow rate | 150 μl/min |
| Column Temperature | 25°C |
| Retention time units | Minutes |
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